摘要
①目的 探讨山东沿海地区自身免疫性甲状腺疾病 (AITDs)的发病与HLA DRB1 0 30 1、DQB1 0 6 0 2等位基因的相关性。②方法 应用多聚酶链反应 序列特异性引物 (PCR SSP)技术 ,对已确诊的Graves病(GD) 90例、桥本甲状腺炎 (HT) 4 3例和正常对照 90例 ,扩增其HLA等位基因DRB1 0 30 1、DQB1 0 6 0 2的目的DNA片段 ,分析两对等位基因在不同人群中表达频率的差异。③结果 山东沿海地区GD和HT病人DRB1 0 30 1等位基因频率均显著高于对照组 (χ2 =4 .6 2 4、5 .0 0 3,P <0 .0 5 ) ;而DQB1 0 6 0 2等位基因频率均显著低于对照组 (χ2 =1 1 .2 0 4、6 .970 ,P <0 .0 1 )。④结论 HLA DRB1 0 30 1可能是山东沿海地区GD和HT的易感基因 ,而DQB1 0 6 0
Objective To investigate the association between DRB1*0301 and DQB1*0602 alleles and autoimmune thyroid diseases in Shandong coastal areas. Methods The HLA DRB1*0301 and DQB1*0602 alleles were detected by polymerase chain reaction with sequence specific primers(PCR SSP) in 90 patients with Graves disease(GD) and 43 patients with Hashimoto thyroiditis(HT),and 90 healthy controls. The frequencies of HLA DRB1*0301 and DQB1*0602 alleles were analyzed. Results The frequency of HLA DRB1*0301 was significantly higher in GD and HT groups than that in the control group. The frequency of HLA DQB1*0602 was significantly lower in GD and HT groups than that in the control group ( χ 2=4.624-11.204;P <0.01, 0.05). Conclusion The HLA DRB1*0301 may be the predisposing gene and HLA DQB1*0602 , the resistance gene of GD and HT in Shandong coastal areas.
出处
《青岛大学医学院学报》
CAS
2004年第3期205-207,共3页
Acta Academiae Medicinae Qingdao Universitatis
