摘要
目的 观察氟伐他汀 (Flu)对博来霉素 (BLM)所致的大鼠肺纤维化的治疗作用 ,探讨治疗肺纤维化的新途径。方法 SD大鼠随机分为 3组 ,每组 2 5只 ,分别为N组 :生理盐水对照组 ;BLM组 :气管内注入含BLMA5的生理盐水 0 2～ 0 3ml(5mg/kg) ;复制肺纤维化动物模型 ;Flu组 (BLMA5+Flu) ,于BLMA5灌注后每天给予Flu 2 0mg/kg灌胃。 3组动物于气管内灌注生理盐水或BLMA5后第 1、3、7、14、2 8天各随机处死 5只。取肺组织 ,进行病理学观察 ;检测肺组织匀浆中羟脯氨酸 (HYP)的变化 ;支气管肺泡灌液(BALF)离心后 ,沉淀细胞测定巨噬细胞、中性粒细胞及淋巴细胞的百分比 ;放免法检测上清液中透明质酸 (HA)、层黏连蛋白 (LN)的变化。结果 病理学观察显示 :Flu组肺泡炎和纤维化程度较BLM组均减轻 ,但仍未恢复到正常水平 ;Flu组从第 7天开始 ,肺组织HYP较BLM组明显降低 (P <0 0 1) ,但仍高于N组 ;BALF中Flu组细胞总数及中性粒细胞、淋巴细胞百分比较BLM组均有所下降 ;BALF中Flu组HA、LN分别在第 3～ 14天和第 3天后显著低于BLM组 (P <0 0 1或P <0 0 5 )。结论 Flu对大鼠实验性肺纤维化有一定治疗作用。
Objective To investigate the therapeutic effect of fluvastatin in bleomycin-induced pulmonary fibrosis in rats. Methods Pulmonary fibrosis was induced in SD rats by intratracheal instillation of bleomycin A_ 5 . The rats were than divided randomly into three groups: the rats in the first group received daily fluvastatin 20mg/kg (group Flu),those of the second group received normal saline (group BLM) orally only,and those of controls (group N) received normal saline both intratracheally and orally. Five rats in each group were sacrificed 1,3,7,14 and 28 days after intratracheal instillation of bleomycin. Pathological changes in the lungs were evaluated by HE stain and Masson′s trichrome stain. Collagen content of the lung tissue was assessed by hydroxyproline concentration. Alveolar macrophages,polymorphonuclear leukocytes and lymphocytes in bronchoalveolar lavage fluid (BALF) were counted. Hyaluronic acid (HA) and laminin (LN) in BALF were determined by radioimmunoassay. Results The degree of alveolitis and pulmonary fibrosis in group Flu was improved as compared with that of group BLM. Hydroxyproline concentrations of group Flu were significantly lower than that of group BLM 7 days after bleomycin A_ 5 instillation. Total cell counts and percentage of polymorphonuclear leukocytes and lymphocytes in BALF were significantly reduced in group Flu. HA and LN levels in BALF were also lower in group Flu compared with group BLM. Conclusion Fluvastatin could alleviate bleomycin-induced pulmonary fibrosis in rats.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2004年第8期703-706,共4页
Medical Journal of Chinese People's Liberation Army
