摘要
目的 研究汉黄芩素对人早幼白血病HL -60细胞端粒酶活性的影响。方法 不同浓度的汉黄芩素作用HL -60细胞后 ,MTT法测细胞的生长和增殖情况 ,TRAP -PCR -ELISA法研究细胞端粒酶活性的变化。结果 汉黄芩素能明显地抑制HL -60细胞的生长 ,且呈时间、浓度依赖性。不同浓度的汉黄芩素能下调HL -60细胞的端粒酶活性 ,且同样呈时间、浓度依赖性。结论 汉黄芩素可抑制HL -60细胞的端粒酶活性 ,可能是其诱导HL
Objective To investigate effect on telemerase of HL-60 cells treated by wogonin.Methods HL-60 cells were treated by wogonin with different concentrations.Then the inhibiting effect and the change of telomerase activity in HL-60 cells were detected by MTT assay and TRAP-PCR-ELISA.Results The proliferation of HL-60 cells treated with wogonin was significantly inhibited,that were density and time dependent.Telomerase activity of HL-60 cells treated with wogonin in different concentrations was significantly inhibited,and the effect were also density and time dependent.Conclusion Wogonin can inhibit the telomerase activity of HL-60 cells.It may be one of important regulatory mechanisms in the apoptosis of HL-60 cell.
出处
《四川医学》
CAS
2004年第8期854-855,共2页
Sichuan Medical Journal
基金
广东省卫生厅资助课题 (A2 0 0 2 4 98)
