摘要
目的 研究单次与分次使用粒细胞集落刺激因子 (G CSF)Filgrastim对供体外周血干细胞的动员效果。方法 将 30例健康供体随机均分为 2组 ,分别接受皮下注射Filgrastim 5 μg/kg,每日 1次或 2 5 μg/kg ,每日 2次动员干细胞 ,共 5天。第 6天始采集干细胞 ,以流式细胞仪分析首次采集物免疫表型。结果 以供体的单位体重计算 ,单次动员组与分次动员组首次采集获得的MNC分别为2 93× 10 8/kg和 4 4 2× 10 8/kg,CD34+ 细胞分别为 2 4 6× 10 6/kg和 4 15× 10 6/kg,CD34+ CD38-细胞分别为 0 2 1× 10 6/kg和 0 73× 10 6/kg,分次动员组均高于单次动员组(P <0 0 5 )。CD34+ DR-细胞分次动员组与单次动员组比较差异无统计学意义 (P >0 0 5 )。结论 分次应用Filgrastim可更有效地动员供体外周血干细胞 。
Objective To compare the results of mobilization of peripheral stem cells of two different G-CSF dosage schedules in 30 healthy donors. Methods In one group 15 healthy subjects received 5μg/kg filgrastim once daily subcutaneously (SC), while in the other group another 15 individuals received 2.5μg/kg of filgrastim twice daily SC. Peripheral blood stem cells were collected after five days of filgrastim administration, and flow cytometric immunophenotyping was performed for the first harvest. Results The MNC count was 2.93×10 8/kg donor body weight in the 5μg/kg Qd group compared with 4.42×10 8/kg in the 2.5μg/kg bid group (P<0.05). The CD34 + or CD34 +CD38 - cell numbers were significantly higher in the 2.5μg/kg bid than 5μg/kg qd group (4.15 vs 2.46×10 6/kg; 0.73 vs 0.21×10 6/kg; P<0.05). The CD34 +DR - cell counts were also found to be higher in the 2.5μg/kg bid than 5μg/kg qd group, but not significantly different statiscally (P>0.05). Conclusion Administration of filgrastim twice a day at 2.5μg/kg instead of once a day at 5μg/kg is more efficient in mobilizing stem cells without obvious side effect.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2004年第7期603-604,共2页
Medical Journal of Chinese People's Liberation Army
基金
全军医药卫生科研"十五"重点项目 (编号 0 1Z0 30 )资助课题
关键词
粒细胞集落刺激因子
干细胞动员
供体
granulocyte colony stimulating factor
stem cell mobilization
donor
