摘要
目的 :探讨 stat3磷酸化 (P- stat3)和 p5 3基因表达在表皮肿瘤发生中的作用。方法 :选择皮肤鳞状细胞癌 (SCC) 30例、基底细胞癌 (BCC)、皮肤脂溢性角化 (SK)和正常皮肤各 2 0例 ,运用免疫组化方法 ,观察细胞中 P- stat3和 p5 3蛋白的表达。结果 :1与正常皮肤和皮肤 SK相比 ,P- stat3在皮肤 SCC、BCC中呈明显的上调表达 (P<0 .0 0 1) ,皮肤 SCC中 P- stat3的表达强度又明显地高于 BCC(P<0 .0 5 ) ;2 P- stat3在皮肤 SCC中的表达强度与肿瘤的分化程度有关 (P<0 .0 5 ) ,阳性表达率与肿瘤浸润的深度有关 (P<0 .0 5 ) ,与肿瘤的大小无关。 3正常皮肤和皮肤 SK无 p5 3的表达 ,皮肤 SCC和 BCC中 p5 3表达明显上调 (P<0 .0 0 1) ,且在皮肤 SCC中 P5 3的表达强度与肿瘤的分化程度有关 (P<0 .0 5 ) ,阳性表达率与肿瘤浸润的深度无关 ,与肿瘤是否位于暴露部位有关 (P<0 .0 5 )。4皮肤 SCC中 ,P- stat3和 p5 3的阳性表达强度具有正相关性 ,rs=0 .6 41,P<0 .0 5。结论 :1P- stat3级联分子的表达异常可能在表皮肿瘤的发生中起重要作用。2 stat3的过度活化可能与皮肤 SCC的侵袭性生长潜能密切相关。3p5 3基因可能与 stat3共同参与皮肤
Objective: To investigate the effect of stat3 phosphorylation and p53 expression on human epidermal non-melanoma cutaneous tumours. Methods: Immunohistochemistry technique was employed to measure the expression of p-stat3 and p53 protein in skin tissue from 30 cases of skin squamous cell carcinoma (SCC), 20 cases of basal cell carcinoma (BCC), 20 cases of seborrhoeic keratosis (SK) and 20 normal subjects. Results: (1) p-stat3 protein was abnormally increased in SCC and BCC as compared with normal skin and SK. Expression of p-stat3 in SCC was also significantly higher than that in BCC. (2) Expression of p-stat3 was higher in poorly-differentiated cancers than that in well-differentiated cancers in SCC. The positive rate of p-stat3 expression was correlated with the depth of tumor invasion, but not with tumor size. (3) There was no p53 protein expression on normal skin and SK, it was significantly upregulated in SCC and BCC. In SCC, the intensity of p53 expression was associated with tumor differentiation. There was no correlation between the positive rate of p53 expression and the depth of tumor invasion, whereas the positive rate of p53 expression was correlated with the sun-exposure area. (4) There existed positive correlation between the expression intensity of p-stat3 and p53 in SCC (r=0.641,P<0.05). Conclusion: (1) The overexpression of p-stat3 may play an important role in the development of epidermal tumors. (2) The abnormal activation of stat3 may be related to metastatic potentials in SCC. (3) Both p53 gene and stat3 may contribute to the pathogenesis of skin SCC.
出处
《浙江大学学报（医学版）》
CAS
CSCD
2004年第4期331-334,共4页
Journal of Zhejiang University(Medical Sciences)
基金
浙江省卫生厅科研基金 (2 0 0 4 A0 4 6 )资助项目
