摘要
研究了在pH4 5的HAc NaAc缓冲介质中 ,利用抗坏血酸对钒催化溴酸钾氧化还原型罗丹明B显色反应具有强烈的活化作用 ,建立了催化动力学光度法测定抗坏血酸的新方法。方法的线性范围为 0～ 7 0 μg·mL- 1 ,检出限为 2 5× 10 - 7g·mL- 1 ,用于维生素C片和西红柿中抗坏血酸的测定 ,结果满意。
In this paper, a new catalytic kinetic spectrophotometric method for the determination of trace amounts of ascorbic acid has been studied. The method is based on the activation effect of ascorbic acid on vanadium( V) catalyzed oxidation of deoxidized rhodamine B by potassium. bromate in weak acidic medium at pH 4.5. The optimal experimental conditions have been discussed. The absorbance was measured at 555 run. The linear range of the method was 0-7.0 mug (.) mL(-1). The detection limit for ascorbic acid was 2.5 x 10(-7) g(.)mL(-1). The method has been applied to the determination of ascorbic acid in vitamin C tablet and tomato with satisfactory results.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2004年第7期873-875,共3页
Spectroscopy and Spectral Analysis
基金
安徽省高校自然科学基金 (99JL0 1 80
2 0 0 1KJ1 96)资助课题
