摘要
目的观察中药复方更年康对老年雌鼠内分泌免疫功能的调节作用。方法选择有骨质疏松的老年雌性大鼠作为研究对象 ,与正常青年雌性大鼠对比 ,分别用中药复方更年康和尼尔雌醇灌胃 3个月 ,观察中药复方更年康对骨质疏松的治疗作用及对内分泌免疫功能的调节作用。采用双能X线吸收技术(DEXA)检测骨密度 ,放免 (RIA)法检测血清雌二醇 (E2 )、卵泡刺激素 (FSH)、黄体生成素 (LH)水平 ,流式细胞术 (flowcytometry)进行脾脏T细胞亚群 (CD4 +、CD8+)及T细胞凋亡的检测。结果老年骨质疏松大鼠骨密度减少 ,血清E2 水平下降 ,FSH、LH水平上升 ,脾脏CD4 +,CD4 +/CD8+明显下降 ,T细胞凋亡率显著上升 ,中药复方更年康能提高骨质疏松大鼠的骨密度 ,能使FSH、LH水平下降 ,而血清E2 水平升高不明显 ,T细胞CD4 +,CD4 +/CD8+升高 ,接近正常水平 ,T细胞凋亡减少。结论中药复方更年康对老年雌性骨质疏松症大鼠有治疗作用 ,并能调节内分泌 ,提高机体免疫力。
ObjectiveTo observe the effect of Compound Gengniankang (GNK) in regulating the endocrine and immune functions in aged female rats. MethodsAged female rats with osteoporosis were selected as the object for observation and healthy young rats were taken for control. Animals were administered by GNK and nilestriol respectively, through gastric perfusion, for 3 months to observe the therapeutic effect of drug treatment on osteoporosis and the regulatory effect on endocrine and immune function. Bone mineral density (BMD) was measured by double energy X ray absorption technique, serum levels of estradiol (E 2), follicule stimulating hormone (FSH) and luteinizing hormone (LH) were determined by RIA, T cell subsets and apoptosis in spleen were detected by flow cytometry. ResultsIn aged rats with osteoporosis, the BMD decreased, serum level of E 2 lowered, FSH and LH levels raised, splenic CD 4 +, CD 4 +/CD 8 + significantly decreased and T cell apoptosis rate significantly elevated. GNK could increase the BMD, lower the FSH and LH levels, but showed no significant effect on E 2 level. It could increase the CD 4 + and CD 4 +/CD 8 + ratio to nearby the normal range, and reduce the apoptosis of T cells. ConclusionGNK has therapeutic effect on osteoporosis in aged rats, and is able to regulate the endocrine and enhance the immune function in organism.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2004年第6期545-548,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
山西省科委资助攻关项目 (No.0 2 2 0 74)
