摘要
目的 :探讨Ⅰ型单纯疱疹病毒 (HSV 1)所致的角膜炎中基质金属蛋白酶 (MMPs)mRNA的表达。方法 :雌性BalB/c小鼠眼角膜接种HSV 1(KOS株 )以诱发单纯疱疹性角膜炎 (HSK)。收集正常角膜及感染后第 2 ,7,14d的感染角膜 ,应用RT PCR技术检测基质金属蛋白酶 2 ,基质金属蛋白酶 9(MMP 2 ,MMP 9)的mRNA表达。结果 :除正常角膜MMP 9mRNA为阴性表达其他各组均获得阳性目的片断。感染后第 2dMMP 2mRNA表达较正常组差异没有显著性 (P >0 .0 5 ) ,感染后第 7dMMP 9mRNA表达与第 2d的表达差异没有显著性 (P >0 .0 5 ) ,其他各组差异均有显著性意义 (P <0 .0 1)且大体上呈随感染天数增加表达增高的趋势。结论 :在实验性HSK中存在基质金属蛋白酶mRNA的表达且MMP 2 ,MMP \|9的mRNA高表达参与了角膜溃疡、角膜新生血管的形成等病变的发生。
Objective: To explore the expression of matrix metalloproteinases (MMPs) mRNA in corneas with infection of herpes simplex virus type-1(HSV-1). Methods: Keratitis was induced in BalB/c mice by inoculating the cornea with HSV-1(KOS strain).Corneas were harvested before infection and at day 2,7 and 14 post-infection. The expression of MMP-2 and MMP-9 mRNA were examined by reverse transcription-polymerase chain reaction (RT-PCR). Results: Each groups showed positive segments except the expression of MMP\|9 mRNA in the normal group. The MMP-2 mRNA at day 2 post-infection did not express significantly compared with the normal group( P> 0.05).The MMP-9 mRNA at day 7 post-infection did not express significantly compared with the 2 day post-infection group( P> 0.05).The differences among other groups were statistically significant ( P< 0.01) and they presented the trend that the expression increased with the time passed post-infection on the whole. Conclusion: The expression of the MMP-2 and MMP-9 mRNA can be detected in HSK cornea. This enzyme was shown to contribute to the ulcerative and neovascularization process that occurs in the corneal in response to HSV infection.
出处
《武汉大学学报（医学版）》
CAS
2004年第4期411-414,共4页
Medical Journal of Wuhan University
