摘要
目的 观察比较前列地尔预处理及经典缺血预处理对在体大鼠缺血 /再灌注心肌损伤的保护效应。方法 动物分为假手术对照组、缺血再灌注组、经典缺血预处理组、前列地尔预处理早期保护组及延迟保护组 ,透射电镜观察心肌超微结构的变化 ,同时用黄嘌呤氧化酶法测定心肌组织的超氧化物歧化酶 (SOD)活性和用硫代巴妥酸法测定丙二醛 (MDA)含量。结果 前列地尔预处理及经典缺血预处理均有保护心肌超微结构和抗氧化效应 ,与模型组相比 ,经典缺血预处理组、前列地尔预处理早期保护组及延迟保护组明显降低缺血再灌注后的心肌MDA含量 (P<0 .0 5 和升高T SOD (P<0 .0 5 水平。结论 前列地尔预处理对在体大鼠缺血 /再灌注心肌损伤具有保护效应 ,能模拟经典缺血预处理心肌保护效应。
Objective To investigate the effects of prostaglandin E 1 pretreatment (PGE 1-P) and ischemic preconditioning(IP)on cardiac ischemic-reperfusion models of rats. Methods Rats were randomly divided into five groups:sham operated group, ischemic-reperfusion model group,ischemic preconditioning group, prostaglandin E 1 early and delayed pretreatment protection group.The myocardium ultratructure was observed under electron microscope and levels of SOD (superoxide dismutase) and MDA (malondialdehyde) in myocardium were measured with xanthine oxidase technique and thio-barbituric acid technique respectively. Results Ischemic preconditioning and prostaglandin E 1 pretreatment have obvious effects on the protection of the ischemic myocardium ultratructure compared with sham operated group. The levels of SOD in ischemic preconditioning and prostaglandin E 1 pretreatment groups were significantly higher than that in sham operated group. The levels of MDA in sham operated group, ischemic preconditioning and prostaglandin E 1 pretreatment groups were significantly lower than that in ischemic-reperfusion group. Conclusion The results indicate that prostaglandin E 1 pretreatment or ischemic preconditioning have some protective effects against ischemia/reperfusion myocardium.
出处
《解剖科学进展》
CAS
2004年第2期133-135,共3页
Progress of Anatomical Sciences
