摘要
应用显微形态观察和变性梯度凝胶电泳 (DGGE)对云南腾冲热海两热泉菌藻席的细菌多样性进行了比较分析。直接从环境样品中提取总DNA ,用两套细菌通用引物进行PCR扩增 ,得到包含V8和V9高变区的 1 6SrDNA片段 ,进行DGGE分析 ,结合形态观察 ,结果显示 ,热泉菌藻席中存在丰富的细菌多样性 。
The microbial mats of two hot springs in Tengchong Rehai of Yunnan, China, were analyzed by microscopy and PCR denaturing gradient gel electrophoresis. Total DNAs directly extracted from environmental samples were amplified by PCR with two sets of bacteria specific primers. The PCR products, which include the V8 and V9 high variable regions respectively, were analyzed using DGGE. By comparing the morphology and DGGE patterns, the results not only indicated rich bacterial diversity profiles but also showed the microbial community differences between the two extreme environments.
出处
《微生物学报》
CAS
CSCD
北大核心
2004年第4期431-435,共5页
Acta Microbiologica Sinica
基金
国家自然科学基金项目 ( 3 0 2 60 0 0 4)~~
