摘要
目的 观察外源性内皮素 1(exET 1)致肺出血作用及外源性降钙素基因相关肽(exCGRP)对ET 1的拮抗作用。方法 实验① :新生Wistar大鼠 10 0只 ,随机分对照组A组 10只 ,实验组B、C、D、E 4组各 2 0只、F组 10只 ,经气管插管于气管内滴入生理盐水 (对照组 )及不同浓度exET 1(实验组 )各 30 μl。实验② :Wistar大鼠 5 0只 ,随机分对照组D1组 10只 ,实验组D2 、D3 、D4、D5组各 10只。于气管内滴入生理盐水 (D1组 )及 4× 10 -6mol/LexET 1(D2 ～D5组 )各 30 μl,30min后D3 ～D5组再滴入不同浓度exCGRP 2 0 μl。③各组滴药 3h后处死动物 ,观察肺组织大体病理及HE染色改变。结果 ①不同浓度exET 1气管内滴入 ,可引起不同程度肺出血 ,其中D组 4× 10 -6mol/LexET 1致肺出血率高达 80 % ,死亡率 2 0 %。②气管内滴入exET 1后 ,再滴入不同浓度exCGRP ,均可减轻exET 1所致肺出血 ,其中 6 7× 10 -6mol/LexCGRP效果最佳 ,肺出血率降至 2 0 % ,死亡率 0 %。结论 exET 1气管内滴入可致肺出血 ,推测肺组织内源性ET 1异常升高 ,是导致肺出血发病机制之一。exCGRP气管内滴入 ,能有效拮抗exET
Objective To observe the phenomenon of pulmonary hemorrhage(PH)induced by exogenous endothelin 1 (exET 1) and the antagonizing effect of exogenous calcitonin gene related peptide (exCGRP) in newborn rats Methods ① To study the exET 1 induced PH: 100 newborn Wistar rats were randomly assigned into control group (group A, n =10) and experiment groups (20 rats in each of groups B, C, D and E and 10 in group F) Thirty μl of normal saline and different concentrations of exET 1 in saline (ranged from 2×10 -6 mol/L to 10×10 -6 mol/L)were dripped into the rats′ trachea through intubation for control group and the experiment groups, respectively ② To study the antagonizing effect of calcitonin gene related peptide against endothelin:50 rats were randomly assigned into control group (group D 1, n =10) and experiment groups D 2、D 3、D 4 and D 5 (10 rats in each group), and were treated with 30 μl of normal saline as control and 4×10 -6 mol/L exET 1 via tracheal dripping Twenty μl of exCGRP (concentrations ranged from 6 7×10 -8 mol/L to 6 7×10 -6 mol/L) were geiven by dripping to rats in groups D 3 to D 5 30 minutes after the administration of exET 1 ③ The rats were sacrificed 3 hours after the first tracheal dripping and the gross anatomical and histological (HE staining) changes in lungs were observed Results ① Following the treatment with exET 1,the rats showed cyanosis and dyspnea rapidly The severity of respiratory symptoms varied in a dose dependent fashion with the concentrations of exET 1. The symptoms were relieved in the survived rats in about 30 minutes The rats of all exET 1 treated groups presented with different degree of PH and group D (treated with 4×10 -6 mol/L of exET 1) had the highest incidence (diffuse PH 30%, focal PH 25%, spotty PH 25% and 80% in total), with a mortality of 20% Rats in group E and F had lower incidence of PH (50% and 20%) but higher mortality (35% and 60%). ② After the adm
出处
《中华儿科杂志》
CAS
CSCD
北大核心
2004年第6期446-449,共4页
Chinese Journal of Pediatrics
基金
广州市计划委员会重大科技攻关项目(穗计科[1998]11号)
