摘要
目的探索新的构建人工神经的方法,寻找合适的支持周围神经移植的基质材料。方法将鼠胚皮质神经元细胞种在PLGA生物膜支架上,用自制的神经轴突延长装置对其延长。观察神经元细胞及轴突生长情况,测定轴突延长后轴膜的渗透性。同时用免疫组化方法对神经微丝蛋白进行染色。结果鼠胚的神经元和神经轴突束可以在PLGA生物膜上延长和继续培养。这些神经元和延长后的轴突束可以保持正常形态和功能。结论这种复合神经元轴突束的三维支架结构具有人工神经的基本特征。本研究为用延长的神经轴突束修复神经缺损打下基础。
Objective To explore a new method to fabricate artificial nerve and to find suitable support matrices for peripheral nervous system (PNS) transplantation. Methods The rat embryo cortical neuronal cells were cultured after they were seeded on PLGA membrane scaffolds, and elongated with the self-made neuro-axon extending device. The growth and morphological changes of the neuron axons were observed and the axolemmal permeability was measured. We also used immunohistochemical techniques to stain neurofilament protein. Results The rat embryo neuron axon could be elongated and cultured on the PLGA membrane. The neuron cells and the axons could remain their normal form and function. Conclusion Our finding that three-dimensional scaffolds with elongated neuron axon have the basic characteristics of the artificial nerve will help the repair of nerve defects using elongated neuron axon.
出处
《中华创伤骨科杂志》
CAS
CSCD
2004年第7期771-773,共3页
Chinese Journal of Orthopaedic Trauma
基金
上海市卫生局立项资助项目(2003-97)
上海市科技发展基金资助项目(014019028)
