摘要
目的探讨谷胱甘肽过氧化物酶1(GPX-1)基因多态性与中国汉族儿童川崎病(kawasaki disease,KD)并冠状动脉损害(CAL)的相关性。方法采用聚合酶链反应-限制性内切酶片段长度多态性分析(PCR-RFLP)检测92例川崎病患儿及108例健康儿童GPX-1基因的-46C/T位点和599C/T位点多态性。结果川崎病组GPX-1基因-46C/T位点CC、CT、TT基因型分布和C、T等位基因频率与正常对照组比较差异无显著性意义(χ2=0.174和0.166,P均>0.05)。川崎病组GPX-1基因599C/T位点CC、CT、TT基因型分布和C、T等位基因频率与正常对照组比较差异亦无显著性意义(χ2=0.429和0.368,P均>0.05)。川崎病患儿合并CAL组与无冠状动脉损害(NCAL)组GPX-1基因-46C/T位点基因型分布和等位基因频率比较差异亦无显著性意义(χ2=0.507和0.487,P均>0.05)。川崎病患儿合并CAL组与NCAL组GPX-1基因599C/T位点基因型分布和等位基因频率比较差异亦无显著性意义(χ2=0.635和0.535,P均>0.05)。结论尚未发现GPX-1基因-46C/T位点多态性以及599C/T位点多态性与川崎病及其CAL的发生存在明显关联性。
Aim To investigate the genetic association of glutathione peroxidase-1( GPX-1) gene polymorphism in patients with kawasaki disease( KD),and coronary artery lesions( CAL). Methods There were 92 patients with KD and 108 healthy subjects in this study. The genotype of locus- 46 C / T and locus 599 C / T of GPX-1 were detected by polymerase chain reaction-restriction fragment length polymorphism( PCR-RFLP). Results For- 46 C / T polymorphism and 599 C / T polymorphism in GPX-1 gene,there were no significant differences between KD patients and the controls in genotype frequencies of CC,CT and TT,and allele frequencies of C and T( χ2= 0. 174 and 0. 166,both P >0. 05; χ2= 0. 429 and 0. 368,both P > 0. 05); For- 46 C / T polymorphism and 599 C / T polymorphism in GPX-1 gene,there were no significant differences between KD patients with CAL and without CAL in genotype and allele frequencies of C and T( χ2= 0. 507 and 0. 487,both P > 0. 05; χ2= 0. 635 and 0. 535,both P > 0. 05). Conclusion No association was found in- 46 C / T polymorphism and 599 C / T polymorphism between GPX-1 gene and the risk of KD or its complication of CAL in this study.
出处
《中国动脉硬化杂志》
CAS
北大核心
2015年第3期290-294,共5页
Chinese Journal of Arteriosclerosis
基金
湖南省自然科学基金(12JJ3108)
