摘要
目的 探讨非小细胞肺癌(NSCLC)患者手术前后血清血管内皮生长因子(VEGF)变化情况.方法 选择120例经病理学确诊,有手术指征且术前未行化疗或放疗的NSCLC患者作为试验组.收集试验组患者手术前3 d内、手术后第1、7、30天的外周血标本.采用酶联免疫吸附实验法(ELISA)行血清VEGF检测.选择60例健康体检者作为对照组.结果 NSCLC患者手术前、术后第1、7、30天的血清VEGF水平分别为:(279.14±44.89)μg/L、(282.70±42.74)μg/L、(353.79±44.55)μg/L、(178.40±43.43)μg/L,均高于对照组的(91.40±16.55)μg/L(P＜0.01);患者不同时间VEGF水平差异均有统计学意义(均P＜0.05);NSCLC患者术前血清VEGF水平与其分期呈正相关(P＜0.01).结论 NSCLC患者血清VEGF水平高于健康体检者,其水平在术后1周内逐渐升高,1个月后降低,并且和肺癌分期呈正相关.
Objective To investigate the serum levels change of vascular endothelial growth factor (VEGF) during operation of non-small-cell lung cancer (NSCLC). Methods 120 cases of NSCLC patient diagnosed by pathology as well as with operation indication were selected as the experimental group. The patients selected were required without any chemotherapy or radiotherapy before operation, besides that, they should have good compliance and free will to be examined. During the process of experiment, 60 cases concluded as healthy in the physical examination were chosen as control group. The correlative information of the experimental group were collected including periphery blood specimen collected in 3 days before the operation, and of the 1st day, 7th day and 30th day after the operation while the periphery blood specimen of control group were collected. The serum levels of VEGF were detected by adopting enzyme linked immunosorbent assay (ELISA) method. Results The serum levels of VEGF in NSCLC patients before operation, of the first postoperative day, of the seventh postoperative day and of the thirtieth postoperative day were significantly higher than that in healthy people (P＜0.01), respectively (279.14±44.89)μg/L, (282.70±42.74) μg/L, (353.79±44.55) μg/L, (178.40±43.43) μg/L and (91.40±16.55) μg/L. The serum levels of VEGF in NSCLC patients showed positive correlation with the stages (P＜0.01). Conclusion The serum levels of VEGF in NSCLC patients scale up. At the same time it shows positive correlation with the stages of the primary tumor. The serum levels of VEGF in NSCLC patients scales up by degrees in one week after the operation, and drop one month later.
出处
《肿瘤研究与临床》
CAS
2008年第5期324-326,共3页
Cancer Research and Clinic
基金
云南省教委基金(04y018c)
昆明医学院青年基金(12-26)
