摘要
目的建立复方益母草胶囊中盐酸水苏碱的HPLC含量测定方法。方法高效液相色谱法,采用Agilent ZORBAXNH2(4.6mm×250mm,5m)色谱柱,流动相为乙腈-水(83∶17),流速为1.0mL.min-1,采用蒸发光散射检测器检测,对数法计算含量。结果盐酸水苏碱对照品线性范围为5.32～15.96 g,r=0.999;平均回收率为98.66%,RSD为1.9%(n=9)。结论本方法快速、简便、重复性好、专属性强且准确度高。
OBJECTIVE To establish an HPLC method to determine the content of Stachydrine hydrochloride in compound motherwort capsules.METHODS The Agilent ZORBAX NH2 column was used.The mobile phase was acetonitrile and water(83∶17).The flow rate was 1.0 mL·min 1.The detector was ELSD.Logarithmic method was used to calculate the content.RESULTS The linear range of reference substance of Stachydrine hydrochloride was 5.32~15.96 μg(r=0.999);The average recovery rate was 98.66% and the relative standard deviation(RSD) was 1.9%(n=9).CONCLUSION The quantitaive method is rapid,simple,highly specific with good repeatability and accuracy.
出处
《中国现代应用药学》
CAS
CSCD
2013年第1期72-74,共3页
Chinese Journal of Modern Applied Pharmacy
