摘要
目的:探讨青年抑郁患者是否存在血小板异常激活以及5-羟色胺载体基因多态性(5-hydroxytryptaminetransporterlinkedpolymorphicregion,5-HTTLPR)在血小板异常激活中的的作用。方法:以酶联免疫吸附实验测定50名青年抑郁患者及30名正常对照的血浆血小板因子4(plateletfactor4,PF4)浓度,以聚合酶链反应检测上述实验对象的5-羟色胺载体的基因多态性,进行对比。结果:抑郁患者的血浆PF4(IU/mL)比正常对照高,差异有显著性意义(7.61±1.62和5.45±0.94,t=4.68,P<0.01);抑郁患者的5-羟色胺载体的基因型构成比与正常对照差异无显著性意义;(χ2=0.16,0.02,P均>0.05);基因型为l/l的抑郁患者的血浆PF4浓度犤(9.09±1.01)IU/mL犦较其他两型的血浆PF4浓度犤(7.44±1.39)IU/mL犦高,两者差异有显著性意义(t=4.15,P<0.05)。结论:抑郁患者存在血小板的异常激活;5-HTTLPR应该不是这种异常激活的启动因素之一,但5-HTTLPR对抑郁障碍的血小板异常激活有促进作用。
AIM:To study whether an abnormal activation of platelet exists in young depressive patients and the effect of 5 hydroxytryptamine transporter linked polymorphic region(5 HTTLPR) in the abnormal activation of platelet. METHODS:The plasma level of platelet factor 4(PF4) was assayed with the enzyme linked immunoadsordent assay(ELISA),and the polymorphism of the 5 HTTLPR with the polymerase chain reaction(PCR) in 50 young depressive patients and 30 normal controls for comparison. RESULTS:The level of plasma PF4(IU/mL) in the depression group(7.61±1.62) was significantly higher than that in the control group(5.45±0.94)(t=4.68,P< 0.01).There was no significant difference in the constituent ratio of 5 HTTLPR between the two groups(χ2=0.16,0.02,P >0.05). Depressive patients with the l/l gene type had higher level of plasma PF4[(9.09±1.01) IU/mL]than those with other gene types[(7.44±1.39)IU/mL],and the difference was significant(t=4.15,P< 0.05). CONCLUSION:An abnormal activation of platelet exists in patients with depression and 5 HTTLPR is not one of the promoting factors of platelet activation,but it has the promoting effect on the abnormal activation of platelet with depressive disorder.
出处
《中国临床康复》
CSCD
2004年第18期3518-3519,共2页
Chinese Journal of Clinical Rehabilitation
