摘要
①目的探讨单纯性肾病综合征（ＳＮＳ）病儿血清可溶性白细胞介素２受体（ｓＩＬ－２Ｒ）的变化特点及其临床意义。②方法采用双抗体夹心ＥＬＩＳＡ法和单克隆抗体间接免疫荧光法，检测２４例ＳＮＳ病儿活动期、近期和远期缓解期及２２例健康儿童血清ｓＩＬ－２Ｒ水平和Ｔ细胞亚群。③结果ＳＮＳ病儿活动期血清ｓＩＬ－２Ｒ水平明显高于近期和远期缓解期及正常对照组（ｔ＝５．６８１～６．３６１，Ｐ＜０．０１），而其他各组间差异无显著性（ｔ＝０．２３３～０．６０１，Ｐ＞０．０５）；活动期血清ｓＩＬ－２Ｒ增高与ＣＤ４细胞百分率和ＣＤ４／ＣＤ８细胞比值呈负相关（ｒ＝－０．４９８，－０．６２５，Ｐ＜０．０５，０．０１），而与ＣＤ８细胞百分率呈正相关（ｒ＝０．５１７，Ｐ＜０．０１）；激素敏感组活动期血清ｓＩＬ－２Ｒ水平明显低于低敏感组（ｔ＝２．２７６，Ｐ＜０．０５），活动期血清ｓＩＬ－２Ｒ水平与尿蛋白阴转时间呈低度正相关（ｒ＝０．４３３，Ｐ＜０．０５）。④结论ＳＮＳ病儿活动期血清ｓＩＬ－２Ｒ水平明显增高，此与Ｔ细胞功能紊乱和ＳＮＳ发病机制密切相关；活动期血清ｓＩＬ－２Ｒ水平可作为判断激素近期疗效的指标。
Objective To discuss the changes and their clinical significances of serum soluble interleukin 2 receptor(sIL 2R) in simple nephrotic syndrome (SNS) in children. Methods The changes of sIL 2R and T cell subset in 24 cases of SNS and 22 healthy children were detected by way of double antibody sandwich ELISA and monoclonal antibody indirect immunofluorescense methods. Results The levels of sIL 2R in acute stage of SNS was significantly higher than that in both early and long term remission stage of SNS and that of control group( t=5.681—6.361, P <0.01),while there was no differece in level of sIL 2R among other groups( t=0.233—0.601,P >0.05).The increase of sIL 2R level in acute stage of SNS had a negative correlation with the chang of CD 4 cell and the ratio of CD 4/CD 8( r=-0.498,-0.625, P <0.05,0.01), and a positive correlation with the chang of CD 8 cell( r=0.517, P <0.01). Serum sIL 2R level in acute stage in group with high response to corticosteroid therapy was significantly lower than that in group with low response to corticosteroid therapy( t=2.276, P <0.05).The level of serum sIL 2R in acute stage had a postive correlation with the duration of proteinuria. Conclusion The level of serum sIL 2R in acute stage of SNS was increased obviously. sIL 2R level in acute stage may be used as an indicator that help to judge the early effect of corticosteroid.
出处
《齐鲁医学杂志》
1998年第2期7-9,共3页
Medical Journal of Qilu
