摘要
一株能利用木糖及半纤维素水解液产乙醇的黄曲霉Aspergillus flavus Z7具有产木聚糖酶的能力。通过单因素试验和正交试验优化了产酶培养基,得到最佳组分为玉米芯2%,尿素0.2%,酵母膏0.25%,K_2HPO_4 0.5%,NaNO_3 0.1%,MgSO_4·7H_2O 0.1%;单因素试验表明,用纱布代替塑料布密封摇瓶封口能显著提高产酶量;Z7在碱性条件下具有更强的产酶性能.在最优条件下发酵,能产生最大木聚糖酶活122.23IU/ml.通过薄层分析,验证了Z7产生的木聚糖酶具有水解木聚糖生成木糖及木寡糖的能力。
A strain of Aspergillus flavus Z7,which can utilize xylose or the hydrolysate of hemicellulose to produce ethanol,also has the ability of xylanase production.The optional component of the culture medium were fixed by the single factor experiment and the orthogonal array tests,and they were organized as follows:com core 2%,urea 0.2%,Yeast extract 0.25%,K_2HPO_4 0.5%,NaNO_30.1%, MgSO_4.7H_2O 0.1%,it was testified by the single factor experiment that sealing the shaking flasks with pledget other than plastic paper can obviously increase the xylanase activity;moreover,Z7 showed better xylanase production ability when in the alkali environment.Under the optional fermentation condition,the maximal xylanase activity 122.23IU/mL was proved.Through the analysis of thin-layer chromatography (TLC),the ability of xylanase from Z7,which can hydrolyze xylan to xylose monomer and oligomer,was vividly displayed.
出处
《生物技术通报》
CAS
CSCD
北大核心
2009年第S1期348-351,363,共5页
Biotechnology Bulletin
基金
国家"863"计划(2007AA100702)
国家科技支撑计划项目(2007BAD78B04)
