摘要
目的建立豆制品和粉干中乌洛托品的气质联用(GC-MS)测定方法。方法样品中乌洛托品经三氯乙酸水溶液提取、阳离子交换柱净化后GC-MS测定。结果该方法的线性范围为0.1～5 mg/kg,相关系数r为0.998;检出限为0.05 mg/kg;回收率为78.5%～97.0%;相对标准偏差(RSD)为5.7%～10.9%。结论该方法准确、操作简单,可用于实际样品中乌洛托品的含量测定。
Objective To establish a qualitative and quantitative method for the determination of urotropine in soybean products and cereal noodles by gas chromatography-mass spectrometry(GC-MS).Methods The sample was extracted by 1.5% trichloroacetic acid aqueous solution.After purified by cation exchange cartridge,the sample solution was detected by GC-MS.Results The method showed a good linearity over the range of 0.1~5 mg/kg with relative coefficient r=0.998.The recovery was 78.5%~97.0% with relative standard deviation(RSD) of 5.7% ～10.9%.The limit of detection was 0.05 mg/kg.Conclusion The method is accurate and easy to operate.It is suitable for the determination of urotropine in real sample.
出处
《食品安全质量检测学报》
CAS
2013年第3期873-876,共4页
Journal of Food Safety and Quality
基金
公益性行业科研专项-"双打"专项(2012104003-6)~~
关键词
乌洛托品
气质联用(GC-MS)
豆制品
粉干
urotropine
gas chromatography-mass spectrometry(GC-MS)
soybean products
cereal noodles
