摘要
目的:探寻可能对丙型肝炎病毒(HCV)有作用的mi-RNA。方法:用体外转录得到的HCV感染正常的Huh-7.5.1细胞,然后加入干扰素作用,采用mi-RNA表达谱芯片技术,分别测定Huh-7.5.1细胞正常组和HCV感染组及干扰素作用组的mi-RNA的表达,采用2(-ΔΔCT)法分析组间microRNA的表达情况。结果:以正常Huh-7.5.1细胞中表达的mi-RNA为基准,经过筛选发现13种mi-RNA在HCV感染后表达上调,而在干扰素治疗后则出现下调;另外还有7种mi-RNA则在HCV感染后出现表达下调,而在干扰素治疗后则发生上调。结论:mi-RNA10a、mi-RNA21、mi-RNA149和mi-RNA152及mi-RNA210可能与HCV的复制转录有关。
Objective: To screen the mi-RNA expression profile after interferon treatment in cells infected with hepatitis C virus(HCV).Methods: Huh-7.5.1 cells was infected with HCV by in vitro transcription and cultured with interferon.The mi-RNA microarray was used to measure the mi-RNA expression in the control group,HCV transcription group and interference group.Intra-group differences were analyzed by the 2(-ΔΔCT) method.Results: With mi-RNA expressed in normal Huh-7.5.1 cells as a benchmark,expressions of 13 kinds of mi-RNAs were up-regulated after HCV infection and then down-regulated following interferon treatment;7 were down-regulated after HCV infection and then up-regulated following interferon treatment.Conclusion: mi-RNA10a,mi-RNA21,mi-RNA149,mi-RNA152 and mi-RNA210 may be related to hepatitis C virus replication and transcription.
出处
《浙江大学学报（医学版）》
CAS
CSCD
北大核心
2011年第6期598-602,共5页
Journal of Zhejiang University(Medical Sciences)
基金
国家“十二五”科技重大专项基金(2012ZX10002-003)
浙江省科技计划基金项目(2009C03011-2)
