摘要
目的:探讨miR-122对α-干扰素(IFN-α)抗丙型肝炎病毒(HCV)效应的影响。方法:给感染HCV的Huh7.5.1予miR-122模拟物(20 nmol/L、100 nmol/L、400 nmol/L)和(或)IFN-α(1 000 IU/ml)处理,采用荧光定量PCR检测HCV RNA水平;给Huh7.5.1细胞感染不同数量HCV(107拷贝,106拷贝,105拷贝)和(或)IFN-α处理,荧光定量PCR检测HCV RNA水平。结果:IFN-α以时间依赖方式抑制HCV复制,在48 h时使HCV RNA下降了83%。miR-122模拟物呈剂量依赖性促进HCV RNA复制(P<0.05)。IFN-α的抗病毒效应与miR-122模拟物水平(20 nmol/L、100nmol/L、400 nmol/L)成反比,与对照组比较有显著性差异(73.3%±3.5%,64.67%±5.5%,56.33%±5.1%vs 84%±4.5%,后两组P<0.05)。IFN-α的抗病毒效应与HCV载量呈反比(105拷贝组vs 107拷贝组,P<0.05)。结论:在细胞感染模型中,miR-122促进HCV的复制,并且,miR-122的表达可部分中和IFN-α的抗HCV效应。
Objective: To investigate the influence of miR-122 on IFN-α treatment for HCV infection.Methods: Huh7.5.1 cells infected with HCV were treated with miR-122 mimics(20 nmol/L,100 nmol/L,400 nmol/L) and/or IFN-α(1 000 IU/ml).The relative expression of HCV RNA was detected by real-time polymerase chain reaction(PCR).Huh7.5.1 cells were treated with different amounts of HCV(107copies,106copies and 105copies) and/or IFN-α(1 000 IU/ml).Results: IFN-α suppressed the replication of HCV in a time-dependent manner,resulting in a ~83% reduction of HCV at 48h.MiR-122 mimics facilitated replication of HCV RNA in a dose-dependent manner(P<0.05).The antiviral effect of IFN-α was inverted to levels of miR-122 mimics(20 nmol/L,100 nmol/L,400 nmol/L),(73.3%±3.5% vs 84%±4.5%,P>0.05;64.67%±5.5% vs 84%±4.5%,P>0.05;56.33%±5.1% vs 84%±4.5%,P<0.05).The antiviral effect of IFN-α was inverted to HCV load(105copies group vs 107copies group,P<0.05).Conclusion: MiR-122 facilitates replication of HCV RNA in the cell culture system;and the expression of miR-122 may partly counteract the anti-HCV effect of IFN-α.
出处
《浙江大学学报（医学版）》
CAS
CSCD
北大核心
2011年第6期588-592,652,共6页
Journal of Zhejiang University(Medical Sciences)
基金
国家"十二五"科技重大专项(2012ZX10002-003)
浙江省科技计划项目(2009C03011-2)
