摘要
目的探讨DACT1在结肠癌中的表达及其对结肠癌细胞SW480增殖、迁移和侵袭的影响。方法 Western blot检测DACT1在结肠癌组织中的表达;CCK-8进行细胞增殖实验;细胞划痕实验检测细胞迁移能力;用Transwell小室法检测细胞转染前后侵袭力改变。结果 21例结肠癌组织中有14例DACT1表达增高,DACT1高表达的SW480增殖能力明显增强(P<0.05),细胞划痕后24h,DACT1高表达的细胞划痕宽度恢复70%,而对照组划痕宽度恢复20%(P<0.05)。实验组SW480细胞侵袭数量明显高于对照组(P<0.05)。结论 DACT1在结肠癌中高表达并促进结肠癌细胞的增殖、迁移和侵袭。
Objective To investigate the expression pattern of DACT1 in human colon cancer and the effect of DACT1 on the proliferation,migration and invasion of SW480.Methods Western blotting was used to examine the DACT1 expression in colon cancer tissues.The proliferation of SW480 cells was quantified by CCK8 assay.The migration and invasion of SW480 was measured with wound healing assay and Transwell test,respectively.Results The expression of DACT1 in colon cancer showed a significant increase compared to the corresponding normal tissue in 14(66.7%) of the 21 patients.Compared with SW480 and vector control cells,the proliferation,migration and invasion of pEGFP-N1-DACT1 transfected SW480 were apparently increased(P<0.05).Conclusions DACT1 is upregulated in human colon cancer and can promote cell proliferation,migration and invasion of SW480.
出处
《中华临床医师杂志（电子版）》
CAS
2011年第12期3432-3435,共4页
Chinese Journal of Clinicians(Electronic Edition)
基金
国家自然科学基金(30770440)
关键词
结肠肿瘤
细胞增殖
细胞运动
肿瘤浸润
DACT1
Colonic neoplasms
Cell proliferation
Cell movement
Neoplasm invasiveness
DACT1
