摘要
Objective To investigate whether the apoptosis is involved in the mechanism of cytotoxicity of trichosanthin to gastric cancer cells. Methods Morphologic studies and TL]AIEL stainning were used to ex-plore qualitatively and quantitatively the apoptotic status of gastric cancer cells before and after treatment with trichosanthin. Results In the experiment in vitro , when gastric cancer cells SGC-7901 were treated by trichosanthin (0. 1μg/ml, 36h), some typical apoptotic morphologic changes occured. A significant increase of apoptotic index (AI) was from (3.78±1.11)%, (3.98±1.12)% and (3.85±1.08)%at 36, 42, 48h to (11.30±2.33)%,(10.22±2.00)% and (11.18±1.85)%,respectively(P<0.01). In the experiment in vivo, nude mice with SGC-7901 xenografted tumor were treated by trichosanthin (0. 3mg kg-1 d -1, × 5d, intraperitoneal injection), some typical apoptotic morphologic changes were observed. These apoptotic cells were limited only in xenografted tumor. Al increased significantly from (4.95± 1 .94) % to (8. 75± 1 .38 )%, (P <0.01). Conclusion The apoptosis would be involved in the mechanism of cytotoxicity of trichosanthin to gastric cancer cells.
Objective To investigate whether the apoptosis is involved in the mechanism of cytotoxicity of trichosanthin to gastric cancer cells. Methods Morphologic studies and TL]AIEL stainning were used to ex-plore qualitatively and quantitatively the apoptotic status of gastric cancer cells before and after treatment with trichosanthin. Results In the experiment in vitro , when gastric cancer cells SGC-7901 were treated by trichosanthin (0. 1μg/ml, 36h), some typical apoptotic morphologic changes occured. A significant increase of apoptotic index (AI) was from (3.78±1.11)%, (3.98±1.12)% and (3.85±1.08)%at 36, 42, 48h to (11.30±2.33)%,(10.22±2.00)% and (11.18±1.85)%,respectively(P<0.01). In the experiment in vivo, nude mice with SGC-7901 xenografted tumor were treated by trichosanthin (0. 3mg kg-1 d -1, × 5d, intraperitoneal injection), some typical apoptotic morphologic changes were observed. These apoptotic cells were limited only in xenografted tumor. Al increased significantly from (4.95± 1 .94) % to (8. 75± 1 .38 )%, (P <0.01). Conclusion The apoptosis would be involved in the mechanism of cytotoxicity of trichosanthin to gastric cancer cells.
