摘要
目的 探讨雌激素受体(ER)基因限制性片段长度多态性(RFLP)与子宫内膜异位症发病的关系。方法 经手术证实的Ⅰ-Ⅳ期子宫内膜异位症患者50例为实验组,随机选择年龄相当的正常妇女50例为对照组,分别提取外周血白细胞DNA,经PCR扩增,PvuⅡ限制性内切酶酶切,琼脂糖电泳观察结果。结果PP、Pp及pp 3种基因型在子宫内膜异位症组出现例数为6、22和22例,而在对照组分别为10,23和17例,两组差异无显著性(P>0.05);等位基因P与p出现频率差异也无显著性(P>0.05)。结论 ER基因型和等位基因频率可能与子宫内膜异位症发病及疾病严重程度无关。
Objective To study the relationship between estrogen receptor (ER) gene restriction fragment length polymorphisms (RFLPs) and endometriosis. Methods 50 Chinese women with endometriosis of stage I ~ IV ER gene RFLPs for PvuII restriction endonuclease were assessed and indicated P. - p ( uppercase letter signifying the absence and lowercase letter the presence of the restriction site) . 50 women without endometriosis were studied as control group. Results There was no significant difference between the patients and the control groups in the frequency of the two-allele PvuII polymorphisms (0.34 vs. 0.43) and in the frequency of PP, Pp and pp genotype (0.12 vs.0.2, 0.44 vs. 0.46, 0.44 vs.0.34, respectively) .Conclusions The genotype and the frequency of the two - allele Pvu II polymorphisms of the estrogen receptor gene probably do not related to the pathogenesis of endometriosis in Chinese people.
出处
《中国妇产科临床杂志》
2001年第2期88-89,98,共4页
Chinese Journal of Clinical Obstetrics and Gynecology
