摘要
目的研究米非司酮对人早孕绒毛组织中转化生长因子β(TGF-β)信号传导通路功能状态的影响以及与终止妊娠之间的关系。方法用免疫组化SP法和RT-PCR方法分别检测药物流产组(20例)和人工流产组(13例)绒毛组织中TGF-β信号传导通路的下游靶基因smad7的蛋白质和mRNA表达水平,结果用平均光密度值和光度比值表示,半定量分析结果。结果药物流产组和人工流产组绒毛组织中smad7的免疫组化结果的平均光密度值分别为0.346 6±0.046 9和0.233 7±0.065 7,P<0.01。药物流产组和人工流产组绒毛组织中smad7mRNA的平均光度比值为0.561 7±0.410 3和0.222 2±0.244 1,P<0.02。结论米非司酮能够增强TGF-β信号传导通路的功能,使该信号通路的下游靶基因纤溶酶原激活物抑制物表达增多,降低了滋养细胞的转移、侵袭能力,可能是该药物致早孕流产的机制之一。
Objective To investigate the effect of mifepristone on the function of transforming growth factor β signal transduction in chorionic villi and the molecular mechanism of mifepristone inducing abortion.Methods Early pregnant chorionic villi were obtained from the surgical aspiration group(n=13) and the mifepristone inducing abortion group(n=20).Expression of smad7 which was the targeting gene of the TGF-β/smads signal transduction pathway in chorionic villi was determined by immunohistochemical staining with...
出处
《山东大学学报（医学版）》
CAS
北大核心
2009年第7期93-95,共3页
Journal of Shandong University:Health Sciences
