摘要
目的:本研究旨在探讨纳洛酮对全脑缺血再灌注损伤后神经细胞的保护作用及机制。方法:选取健康成年雄性Wistar大鼠36只。随机分为假手术组,对照组及治疗组。采用大鼠四条血管阻断方法制备大鼠全脑缺血再灌注模型。治疗组于不同开始时间点累积给药纳洛酮。并于缺血再灌注后48小时处死。采用流式细胞分析技术(Fcm)观察海马区细胞凋亡的变化及Bcl-2、Bax的蛋白表达水平。结果:Fcm标记凋亡细胞的变化:治疗组均与对照组有显著统计学差异(P<0.01)。Bcl-2、bax检查表明治疗组与对照组有显著统计学差异(P<0.01)。结论:纳洛酮可减少脑细胞凋亡的发生,这其中可能与纳洛酮早期上调Bcl-2蛋白的表达或通过减轻Bax对Bcl-2活性的抑制从而降低细胞凋亡的发生有关。
Objective:The aim of this study was to observe the protective effect of Naloxone on the neural apoptosis after global cerebral ischemic-reperfusion injury in rats.Methods:Thirty-six male Wistar rats were used in this study..All rats were divided into three groups at random.Four-vessel occlusion was used to establish the model of global cerebral ischemia reperfusion in the study groups and control group.The Naloxone was injected into abdomen on the different time-spot in treated groups.On the 48h time-spot a...
出处
《脑与神经疾病杂志》
2008年第5期591-594,共4页
Journal of Brain and Nervous Diseases
