摘要
培养大鼠胰岛素瘤细胞(INS-1),以四氧嘧啶(AXN)损伤细胞,培养液中加入不同浓度的多糖AMP-1,检测不同浓度AMP-1对INS-1细胞葡萄糖刺激的胰岛素和C肽分泌量的影响,同时检测细胞存活率。结果表明,一定浓度范围AMP-1对AXN损伤的INS-1细胞分泌胰岛素和C肽均具有一定的促进作用,尤其是在葡萄糖刺激浓度为16.7mmol·L-1时,效果显著;AMP-1可减少AXN对INS-1细胞的损伤,使INS-1细胞存活率增加。
Graded concentrations of AMP-1 were added into the culture medium of INS-1 cells injured by alloxan. The amount of glucose-stimulated insulin and C-peptide secretions in different concentration of AMP-1 was determined and the survival rate was measured. The results showed that graded concentrations of AMP-1 could promote glucose-stimulated insulin and C-peptide secretions in INS-1 cells, especially in the concentration of 16.7 mmol·L -1 of glucose. AMP -1 could reduce the injuries induced by alloxan in INS-...
出处
《中国食用菌》
北大核心
2009年第1期39-41,44,共4页
Edible Fungi of China
基金
国家自然科学基金项目(3040066)
安徽省教育厅自然基金项目(2003kj021
2002kj017)。
