摘要
目的:构建多房棘球绦虫重组质粒BCG-EmⅡ/3-10,探索泡球蚴病防治的新途径。方法:超声粉碎泡球蚴组织提取总RNA,通过RT-PCR扩增EmⅡ/3-10抗原编码基因,将该基因定向克隆到大肠埃希菌-分枝杆菌穿梭表达载体pBCG,构建重组质粒pBCG-EmⅡ/3-10,电穿孔法将该质粒导入BCG构建多房棘球绦虫重组BCG-EmⅡ/3-10。结果:经过PCR扩增鉴定、电泳证实获得的重组基因的大小和插入方向正确。结论:成功构建了多房棘球绦虫重组质粒BCG-EmⅡ/3-10,为进一步研究多房棘球绦虫重组疫苗奠定基础。
Objective:To construct recombinant plasmid pBCG-Em Ⅱ/3-10 of Echinococcus multilocularis and explore a new method for prevention and cure of alveococcosis.Methods:The total RNA was extracted by ultrasound-breaking from alveolar hydatid cyst.The Em Ⅱ/3-10 antigen gene was amplified by RT-PCR from the total RNA and then was cloned into E.coli-Mycobacterium shuttle plasmid pBCG to construct recombinant plasmid pBCG-Em Ⅱ/3-10.The plasmid was introduced into BCG by electroporation to construct rBCG-Em Ⅱ/3-10.Res...
出处
《陕西医学杂志》
CAS
北大核心
2008年第11期1446-1448,共3页
Shaanxi Medical Journal
基金
湖北省教育厅科研基金资助项目(2004D011)
