摘要
目的:研究以HPLC法同时测定乐肝清炎胶囊中栀子苷、甘草素含量的方法。方法:采用Kromasil C18柱(4.6 mm×250 mm,5μm),以甲醇-0.5%冰乙酸为流动相,梯度洗脱,体积流量:1.0 mL·min-1,λ1=238 nm(检栀子苷),λ2=276 nm(检甘草素),柱温30℃。结果:栀子苷在(54.1~162.4)μg·mL-1与峰面积呈良好的线性关系,r=0.999 8,甘草素在(5.2~15.5)μg·mL-1与峰面积呈良好的线性关系,r=0.999 9。平均加样回收率(n=9):栀子苷为99.66%;甘草素为99.90%。结论:本法操作简便,结果可靠,重现性好,可作为该产品质量控制的方法。
Objective:To establish a HPLC method for determination of geniposide and liquiritigenin in Legan-Qingyan Capsules.Methods: A Kromasil C_18 Column(4.6 mm×250 mm,5 μm)was used.The mobile phase was methanol-0.5% glacial acetic acid,the flow rate was 1.0 mL·min-1,λ_1=238 nm(for the geniposide),λ_2=276 nm(for the liquiritigenin),the column temperature was 30 ℃.Results: The linear range for geniposide was within(54.1～162.4)μg·mL-1,r=0.999 8,recovery was 99.66%(n=9).The linear range for liquiritigenin was within(5...
出处
《中国实验方剂学杂志》
CAS
2008年第10期8-10,共3页
Chinese Journal of Experimental Traditional Medical Formulae
