摘要
SD大鼠为模型,在背部四肢关节处做切口,处理方法分为4种:空白对照组(切口两侧皮下不注入任何液体)、模拟对照组(注入药物溶剂三蒸水)、氯化镧组(50mmol·L-10 25ml)和抗TGF β1抗体组(50μg/切口)。用RT PCR的方法,检测各组组织中纤维连接蛋白(FN)mR NA的表达量。结果发现,在伤后14d,模拟组FNmRNA表达量增加(P<0 05),伤后28d,各组之间差异无意义(P=0 05)。氯化镧抑制瘢痕形成的机理可能与FNmRNA的表达无关。
Four fullthickness linear incisions were made on dorsal skin of 20 SD rats One of wound (control) was unmanipulated; the second wound (shamcontrol) was injected with distilled water; the third wound (experimental) was injected with a 50 mmol·L-1 of lanthanum chloride, and the fourth wound (positive control) was injected with 50 μg neutralizing antibody to TGFβ1 Each injection was made up to 025 ml with distilled water Expression of FN mRNA in wound tissue was assayed by RTPCR after 14 and 28 d Shamcontrol group showed the higher expression than other groups after 14 d, no evident differences were found between the groups after 28 d It is possible that lanthanum chloride does not have clear influence on expression of FN mRNA in wound tissue
出处
《中国稀土学报》
CAS
CSCD
北大核心
2002年第z2期270-272,共3页
Journal of the Chinese Society of Rare Earths
关键词
稀土
氯化镧
瘢痕形成
FN
rare earths
lanthanum chloride
scarring
FN
