摘要
目的 测定甘西鼠尾草中丹参酮ⅡA的含量.方法 采用HPLC法,色谱柱:ZORBAX Eclipe XDB-C18(250×4.6mm,5μm)柱, 流动相为:甲醇-水(75:25v/v), 检测波长:270nm.结果 丹参酮ⅡA在6.0～100.0μg·ml-1范围内,峰面积与进样浓度线性关系良好,回归方程Y=186.3+245.1C, r=0.9974,平均回收率为98.0%,RSD=1.67% ; 栽培品甘西鼠尾草中丹参酮ⅡA含量: 1年生 0.305% , 2年生0.539% , 3年生0.776% , 野生品0.573%.结论 所测样品中丹参酮ⅡA含量均高于<中国药典>2005年版丹参项下规定要求, 且栽培3年生甘西鼠尾草其丹参酮ⅡA含量高于野生品.
Objective To determine the contents of tanshinone IIA in the Salvia Przewalskii.Methods The concentration of tanshinone IIA was determined by HPLC method,the analysis involved a 250×4.6mm column packed with C18(5μm).The mobile phase consisted of methyl methanol-water(75:25v/v),the detection was set at 270nm.Results A good linearity was obtained in the range of 6.0-100.0μg·ml-1,the linearegression equation was Y=186.3+245.1C,r=0.9974,the average recoveries was 98.0%,RSD=1.67%;To determine the contents of tanshinone IIA about Salvia Przewalskii showed that: one year grow(0.305%;)two years grow 0.539%;three years grow 0.776%;wild strain grow 0.573% respectively.Conclusion The tanshinone IIA contents in determined Salvia Przewalskii were higher than prescribed contents of Chp(2005),the tanshinone IIA content in tree years grow was higher than that of wild strain.
出处
《青海医学院学报》
CAS
2006年第4期262-264,共3页
Journal of Qinghai Medical College
