摘要
为了提高基因芯片技术的推广应用,研制了一种集PCR扩增、杂交、清洗和检测分析于一体的新型仪器;可以实现快速PCR,30个扩增循环可以在15min内完成。杂交及冲洗在计算机控制下自动完成,避免了人工干预以及环境变化所造成的误差。检测部分采用汞弧灯作为激发光源,CCD采集荧光信号,配合信号识别分析软件自动识别基因芯片信息。
To advance generalization and application of genechips,a new instrument is developed.The instrument integrated the abilities of PCR amplification,crossing,cleaning and testing.It can implement fast PCR which 30 amplifications circulation are completed in 15 minutes.Crossing and cleaning are completed automatic in computer controlling and the error introduced by decision-maker and condition changing is avoided.In testing part,Mercury lamp illuminator is regarded as excitation source,CCD acquiring fluorescence signal and the information in genechips are identified automatic with the signal identifying software.
出处
《仪器仪表学报》
EI
CAS
CSCD
北大核心
2006年第z1期209-211,共3页
Chinese Journal of Scientific Instrument
关键词
基因芯片
温度控制
杂交
自动检测
genechips temperature controlling cross automated inspection
