摘要
目的研究早期、长期辅助运用复方丹参滴丸对自发性高血压大鼠血管壁重建的干预及可能机制。方法10周龄自发性高血压大鼠48只随机分成6组:治疗前对照组、阴性对照组、络活喜治疗组、复方丹参滴丸后期治疗组、复方丹参滴丸早期治疗组和复方丹参滴丸长期治疗组,检测胸主动脉壁厚度和血管壁平滑肌细胞增长率,以及基质金属蛋白酶2、内皮素1和层粘连蛋白相对表达水平。结果与复方丹参滴丸早期治疗组比,复方丹参滴丸长期治疗组血管平滑肌细胞增长率明显减少,而复方丹参滴丸后期治疗组则明显增加。在降压治疗(络活喜)基础上加用复方丹参组其内皮素1、基质金属蛋白酶2表达均有不同程度减少,而层粘连蛋白增加,且出现时间在血管平滑肌细胞增长率变化之前。结论在有效降压治疗基础上早期、长期联合运用复方丹参滴丸可能通过产生生物学效应(基质金属蛋白酶2和内皮素1表达减少,层粘连蛋白表达增加)来遏制因此而产生的力学效应(血管平滑肌细胞增长率),从而改善血管壁的重建和增厚。
Aim To investigate the characteristic and the possible mechanism of vascular remodeling in spontaneously hypertensive rats(SHR) and effect of Fufang Danshen Diwan, when Fufang Danshen Diwan was taken on early stage for long time. Methods Forty-eight ten-week-old SHR were randomly divided into six groups: the control group before treatment (n=8), the negative group (n=8) which were fed with 0.9% sodium chloride for 8 weeks, the amlodipine treatment group (n=8) which were fed with amlodipine for 8 weeks, Fufang Danshen Diwan later treatment group (n=8) which were fed with amlodipine for 8 weeks and then added with Fufang Danshen Diwan for 8 weeks again, Fufang Danshen Diwan early treatment group (n=8) which were fed with amlodipine and Fufang Danshen Diwan for 8 weeks, Fufang Danshen Diwan long-term treatment group (n=8) which were fed with amlodipine and Fufang Danshen Diwan for 16 weeks and then were executed. All the rats were taken blood pressure before they were executed, and then vascular wall of thoracic aorta were taken out, then the wall thickness (WT) and growth rate of vascular smooth muscle cell (VSMC), the expression of matrix metallopro-teinase-2 (MMP-2), endothelin-1 (ET-1) and laminin (LN) were observed. Results Compared with Fufang Danshen Diwan early treatment group, the VSMC growth rate of Fufang Danshen Diwan long-term treatment group descended obviously, nevertheless, that of Fufang Danshen Diwan later treatment group ascended significantly. Based on lowering blood pressure taking Fufang Danshen Diwan earlier and longer would descended the expression of MMP-2 and ET-1 and ascended the expression of LN, which occurred before VSMC growth rate changed. Conclusion Based on lowering blood pressure taking Fufang Danshen Diwan on early stage for long time would restrain vascular remodeling by altering biological effect (descended the expression of MMP-2 and ET-1, ascended the expression of LN), as well as mechanic effect (the growth rate of VSMC).
出处
《中国动脉硬化杂志》
CAS
CSCD
2007年第3期181-184,共4页
Chinese Journal of Arteriosclerosis
基金
浙江省中医药研究课题(2002C016)
