摘要
目的:探讨不同浓度的姜黄素(Cur)对大鼠脑缺血再灌注损伤的保护作用及其机制。方法:采用线栓法阻塞大鼠大脑中动脉(MCAO),建立大鼠局灶性脑缺血实验模型,观察不同浓度姜黄素(20 mg/kg、40 mg/kg、60 mg/kg)对脑缺血再灌注损伤后大鼠神经学评分、脑梗死体积的改变,并用免疫组化法检测热休克蛋白70(HSP70)的表达情况。结果:假手术组无神经行为改变;各用药组神经学评分明显低于缺血再灌组。各用药组脑梗死体积明显小于缺血再灌组;Cur 20 mg/kg组明显大于其它两组。各用药组HSP70阳性率较缺血再灌注组明显增多;Cur 40 mg/kg组优于其它两组。结论:姜黄素对大鼠局灶性脑缺血再灌注损伤有保护作用;其机制可能与增加脑缺血再灌注损伤时HSP70的表达,增强其对神经细胞的保护作用有关。
Objective:To explore the protective effect of curcumin(Cur) on the injury caused by focal cerebral ischemia/reperfusion(I/R) of rats and its mechanism.Methods:The model of middle cerebral artery occlusion(MCAO) and reperfusion was set up.Cur of 20 mg/kg,40 mg/kg and 60 mg/kg was intraperitoneally injected into the rats respectively,and the changes of the neurologic deficiency scores and the infarct volume were observed.The expression of HSP70 in brain tissue was detected by the method of immuno-histochemistry.Results:Sham-operated group presented no neurologic changes.The neurologic deficiency scores in Cur groups were obviously elevated,compared with those in I/R group.The infarct volume in Cur groups was obviously less than that of I/R group,and the infarct volume of Cur 20 mg/kg group was obviously larger than that of the other two groups.Compared with I/R group,the number and the rate of HSP70 positive cells increased obviously in Cur groups,and Cur 40 mg/kg group was supior to the other two groups.Conclusions:Cur can prevent rats from focal cerebral ischemia/reperfusion damage.The mechanism may be that Cur can elevate the expression of HSP70 in local cerebral ischemia/reperfusion damage,thus protecting the neurons more effectively.
出处
《蚌埠医学院学报》
CAS
2007年第6期637-639,封3,共4页
Journal of Bengbu Medical College
关键词
脑缺血
再灌注
姜黄素
栓塞
大脑中动脉
梗死体积
热休克蛋白70
大鼠
cerebral ischemia
reperfusion
curcumin
embolism
middle cerebral artery
infarct volume
heat shock protein 70
rats
