摘要
本文采用乙酰胆碱酯酶(AChE)组织化学、脑损毁和氟磷酸二异丙酯(DFP)预处理技术,研究了黑斑蛙和家鸽顶盖峡核系统的AChE染色图式。这两种动物的顶盖AChE深染色层,基本与视网膜顶盖投射一致。蛙峡核的AChE染色可分成3个区,其中背外侧区染色最深;鸽峡核小细胞部(1pc)和大细胞部(Imc)染色深而均匀。局部损毁顶盖后,蛙峡核和鸽Ipc在对应区域内,AChE染色减弱或消失。DFP预处理表明,鸽顶盖的Ⅲ层细胞含丰富的AChE,其他层细胞和蛙顶盖细胞含有中等和低浓度AChE。峡核细胞含高浓度AChE。在其顶盖损毁的鸽中,对应Ipe区内的AChE染色细胞数量减少,染色变淡。这些结果提示在两栖类和鸟类中,顶盖峡核投射和峡核顶盖投射可能是胆碱能通路。
This paper,using acetylcholinesterase(AChE)histochemistry,brain-lesion and di-isopropylfluorophosphate(DFP)pretreatment techniques,studies AChE distribution in the tectum-nucleus isthmi systems in frogs and pigeons.In these two species of animals,the tectal laminae densely stained for AChE are roughly coincided with the retinotectal projections.Staining pattern in the frog nucleus isthmi consists of 3 areas with different staining intensities.The dorsolateral area being most densely stained.In pigeons,the nucleus isthmi pars parvocellularis(Ipc)and pars magnocellularis(Imc)are densely and evenly stained.Following local lesion of tectum,stainings in the frog nucleus isthmi and pigeon Ipc are topographically reduced or disappeared.DFP-treatment shows that tectal cells,with the exception of cells in layer III containing rich AChE,have moderate or low concentrations of AChE.The isthmic cells are rich in AChE.In the tectum-lesioned pigeons,AChE-stained isthmic cells in the topographical area are reduced in number and paler in color.These results suggest that in amphibians and birds both the tectoisthmic projection and the isthmotectal projection could be cholinergic pathways in nature.
作者
李铮
王书荣
许红艳
颜坤
Li Zheng;Wang Shu-rong;Xu Hongyan;Yan Kun(Institute of Biophysics,Academia Sinica,Beijing)
出处
《解剖学报》
CAS
1987年第1期44-47,119-120,共6页
Acta Anatomica Sinica
