摘要
本实验以15~16期的黑斑蛙胚胎为材料,采用切除眼泡,或切除眼泡后填补一块前脑侧壁组织,或将眼泡连同部分前脑侧壁组织一并切下并旋转180度等显微手术,使预定水晶体外胚层与前脑侧壁组织接触。结果表明,前脑侧壁接触了预定水晶体以后,由于受到后者的诱导作用,接触部位的脑壁组织能转化为次生眼杯。在282个手术例中,计有81例出现次生眼杯,成功率达28.8%。本文对次生眼杯的成因及某些规律进行了讨论,并对负反应的某些原因作了分析。
Three types of microscopic operations were used in this study:1.excision of the optic vesicle alone;2.after excising the optic vesicle,a piece of forebrain tissue taken from another donor was inserted in between presumptive lens ectoderm and forebrain wall;3.cut down the optic vesicle and portion of forebrain tissue and replaced them back in situ by turning over 180°.The operations were carried out on Rana nigromaculata at 15 and 16 stages,in order to bring the presumptive lens ectoderm to come into contact directly with the forebrain tissue for the purpose to analyse the possibility of eye cup formation from the brain wall.The results indicate that the forebrain wall which came into contact with the presumptive lens ectoderm could be induced and differentiated into a secondary eye cup or retina.A total of 81 cases of secondary eye cups among 282 operations(28.8%)were observed.The mechanism for the induction and its significance were discussed.
作者
李新人
余启祥
李桃生
王希成
Li Xinren;Yu Qixiang;Li Taosheng;Wang Xicheng(Department of Biology,Nanjing University,Nanjing)
出处
《解剖学报》
CAS
1987年第4期443-446,468,共5页
Acta Anatomica Sinica
关键词
次生眼杯形成
预定水晶体
诱导
蛙胚
Formation of secondary optic cup
Presumptive lens
Induction
Frog embryos
