摘要
目的探讨不同剂量清热解毒方对干燥综合征模型小鼠颌下腺结构和功能的影响。方法将50只12周龄SPF级雌性非肥胖性糖尿病(NOD)小鼠随机分为中药治疗高剂量组、中药治疗中剂量组、中药治疗低剂量组、西药对照组和空白对照组,分别给予高剂量清热解毒方、中剂量清热解毒方、低剂量清热解毒方、羟基氯喹片、生理盐水干预28 d;并选用10只ICR小鼠为正常对照组,不做处理。对小鼠饮水量、唾液流率、颌下腺指数,及外周血补体C3、补体C4、免疫球蛋白IgG进行分析,同时用免疫组化法观察小鼠颌下腺淋巴细胞浸润情况。结果治疗后,中药治疗高剂量组小鼠体质量明显低于其他各组,死亡率明显高于其他组(P<0.05);中药治疗中、低剂量组小鼠较治疗前饮水量无明显增加(P>0.05),中药治疗高剂量组、西药对照组及空白对照组较治疗前饮水量明显增加(P<0.05),且治疗后中药治疗中、低剂量组小鼠饮水量较西药对照组及空白对照组比较明显减少,差异有统计学意义(均P<0.05)。中药治疗中剂量组唾液流率及颌下腺指数均明显高于空白对照组(均P<0.05),中药治疗高、低剂量组及西药组唾液流率及颌下腺指数与空白对照组比较,差异无统计学意义(P>0.05)。中药治疗各剂量组和西药对照组颌下腺淋巴细胞浸润分级均与空白对照组比较,差异无统计学意义(P>0.05),中药治疗中剂量组颌下腺指数明显高于西药对照组(P<0.01),余中药治疗组颌下腺指数与西药对照组比较,差异无统计学意义(P>0.05);中药治疗高剂量组CXCL13浓度明显低于空白对照组和西药对照组(均P<0.01),余中药组较空白对照组及西药对照组差异均无统计学意义(P>0.05)。治疗后中药治疗低剂量组及空白对照组小鼠血清IgG浓度明显高于正常对照组(P<0.05),余各组较正常对照组差异无统计学意义(P>0.05);中药治疗高剂量组及西药治疗�
Objective To observe the effects of different doses of Qingre Jiedu Recipe(清热解毒方)on the structure and function of submandibular gland in Sjögren′s syndrome model mice.Methods Qingre Jiedu Recipe high-dose group,Qingre Jiedu Recipe medium-dose group,Qingre Jiedu Recipe low-dose group,western medicine control group and blank control group were established with 1012-week-old SPF grade female non-obese diabetic(NOD)mice in each group,which were treated with high dose Qingre Jiedu Recipe,medium dose Qingre Jiedu Recipe,low dose Qingre Jiedu Recipe,hydroxychloroquine and physiological saline,respectively.Ten ICR mice were selected as the normal control group without any treatment.After 28 days of intervention,water intake,salivary flow rate,submandibular gland index,and the levels of C3,C4 and IgG in peripheral blood were analyzed.Meanwhile,the lymphocyte infiltration in the submandibular gland of mice was observed by immunohistochemical method.Results After treatment,the body weight of mice in the Qingre Jiedu Recipe high-dose group was significantly lower than that in the other groups,and the mortality rate was significantly higher than that in the other groups(P<0.05).There was no significant increase in the water intake of Qingre Jiedu Recipe medium-dose group and low dose group(P>0.05),but increased in other groups(P<0.05).The water intake of mice in the Qingre Jiedu Recipe low-dose and medium-dose groups were significantly lower than that in the western medicine control group and the blank control group after treatment(P<0.05).The salivary flow rate and submandibular gland index in the Qingre Jiedu Recipe medium-dose group were significantly higher than those in the blank control group(P<0.05).There was no significant difference in the classification of lymphocyte infiltration in submandibular gland among the Qingre Jiedu Recipe groups,the western medicine control group and the blank control group(P>0.05).The submandibular gland index of non Qingre Jiedu Recipe groups but Qingre Jiedu Recipe medium-d
作者
李奔
李永明
薛鸾
LI Ben;LI Yongming;XUE Luan(Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China)
出处
《辽宁中医杂志》
CAS
2022年第2期204-208,共5页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金(81573925)
上海市卫生健康委员会科研课题(20194Y0180)
