摘要
目的分析蒲地蓝消炎口服液的有效活性成分及其治疗急性咽炎的分子机制。方法分别通过中药系统药理学数据库分析平台(TCMSP)数据库、Genecards数据库查找蒲地蓝消炎口服液中4味中药的有效活性成分及其靶点蛋白和人类急性咽炎相关的作用靶点,构建有效活性成分-疾病靶点的网络图;蛋白互作网络通过String数据库进行构建,通过Metascape数据库进行GO和KEGG富集分析;最后使用分子对接技术对有效活性成分和靶点进行验证。结果经过筛选,蒲地蓝消炎口服液中的槲皮素、β-谷甾醇、汉黄芩素、刺槐黄素以及豆甾醇等多个关键活性成分,可能通过作用在细胞肿瘤抗原p53(cellular tumor antigen p53,TP53)、白细胞介素6(interleukin-6,IL-6)、白细胞介素8(interleukin-8,CXCL8)、过氧化物合酶2(prostaglandin G/H synthase 2,PTGS2)、白细胞介素10(interleukin-10,IL-10)、肿瘤坏死因子(tumor necrosis factor,TNF)等核心基因表达的蛋白上参与急性咽炎的治疗。KEGG富集分析所涉及的通路包括NF-kappaB信号通路(NF-kappa B signaling pathway)、癌症通路(Pathways in cancer)、HIF-1信号通路(HIF-1 signaling pathway)、TRP通道中炎症介质调节(inflammatory mediator regulation of TRP channels)等。结论蒲地蓝消炎口服液可通过多成分、多靶点、多通路的复杂作用机制发挥抗炎作用,进而对急性咽炎起到治疗作用。
Objective To search the molecular mechanism of Pudilan Oral Solutions(蒲地蓝消炎口服液)treating acute pharyngitis.Methods The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform and Genecards database was used to screen out potential chemically active substances and the targets related to human acute pharyngitis separately.Then,the network of active substances and disease targets was pictured.PPI core genes were calculated by String database platform.Perform GO enrichment analysis and KEGG enrichment analysis were carried out by Metascape database.Results After screening,many key chemical components in Pudilan Oral Solutions might be effective for acute pharyngitis treatment through cellular tumor antigen p53(TP53),interleukin-6(IL-6),interleukin-8(CXCL8),prostaglandin G/H synthase 2(PTGS2),interleukin-10(IL-10)and tumor necrosis factor(TNF),namely quercetin,beta-sitosterol,wogonin,acacetin,Stigmasterol,etc.KEGG enrichment analysis results included NF-kappa B signaling pathway,Pathways in cancer,HIF-1 signaling pathway,inflammatory mediator regulation of TRP channels.Conclusion Pudilan Oral Solutions can treat acute pharyngitis through the complex action mechanism of multiple components,multiple targets and multiple pathways by anti-inflammatory.
作者
杨雪
范秀东
任晓楠
庞敏
YANG Xue;FAN Xiudong;REN Xiaonan;PANG Min(The Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110034,Liaoning,China)
出处
《辽宁中医杂志》
CAS
2022年第9期157-161,226-227,共7页
Liaoning Journal of Traditional Chinese Medicine
基金
国家重点研发计划(2018YFC1707410)
关键词
蒲地蓝消炎口服液
急性咽炎
网络药理
分子对接
作用机制
Pudilan Oral Solutions(蒲地蓝消炎口服液)
acute pharyngitis
network pharmacology
molecular docking
mechanism of action
