摘要
荧光探针技术是指用荧光光敏分子对待测物质进行标记,从而实现对待测物定性定量分析的一种技术手段。氟离子是人体重要的微量元素之一,适当摄入氟离子有助于预防蛀牙和骨质疏松症,而过量摄入氟离子会引起多种疾病,因此开发能够检测生物体内氟离子的荧光探针具有重要意义。以教师最新科研成果转换为实验教学内容,设计了“荧光探针的合成及水相中氟离子检测的综合性实验”。通过实验的实施,学生的有机合成能力、光学分析仪器的操作能力以及实验数据分析等能力均得到较大提高。
Fluorescence probe technology refers to a technical means of labeling the substance to be measured with fluorescence probe,enabling qualitative and quantitative analysis of the measured substance.Fluoride ion is one of the important trace elements in human body,proper intake of fluoride ion can help prevent tooth decay and osteoporosis,while excessive intake of fluoride ion can cause a variety of diseases.Therefore,it is of great significance to develop fluorescence probes that can detect fluoride ions in organisms.The latest scientific research results were converted into comprehensive designing experiment“the synthesis of fluorescence probe and detection of fluorine ions in aqueous phase”.Through the implementation of the experiment,the students’ability of organic synthesis,the operation of optical analytical instruments and the analysis of experimental data will be greatly improved.
作者
李新民
杨明焱
吕佳佳
吴庆
袁泽利
LI Xin-Min;YANG Ming-Yan;LV Jia-Jia;WU Qing;YUAN Ze-Li(School of Pharmacy,Zunyi Medical University,Zunyi 563000,China)
出处
《实验室检测》
2024年第5期152-155,共4页
Laboratory Testing
基金
遵义医科大学教育教学改革计划项目(XJJG2018-06-088)
贵州省高等学校金师及金课建设项目
关键词
荧光探针
氟离子
综合设计性实验
fluorescence probe
fluoride ion
comprehensive design experiment
