摘要
目的探究氧化苦参碱靶向抑制Th17分化对小鼠肠道黏膜免疫功能损伤的保护作用,并初步探讨其保护机制。方法30只ICR小鼠,随机分为3组:空白对照组、损伤对照组、治疗组。采用腹腔注射环磷酰胺构建肠道粘膜免疫屏障损伤小鼠模型。记录小鼠在实验期间的精神状态、活动情况;制备小肠切片并进行组织学观察,统计绒毛长度、隐窝深度并统计两者比值;流式细胞术分析肠粘膜组织中Th17,Treg的百分比含量,以及不同时期Th17/Treg的比例;免疫印迹法检测Th17细胞特异性转录因子RORγt、Treg细胞特异性转录因子Foxp3的表达;酶联免疫法(ELISA)检测肠黏膜SIgA的含量。结果与对照组相比,氧化苦参碱可显著上调绒毛长度/隐窝深度比,改善环磷酰胺所致小鼠肠道机械屏障受损状况;降低不同时期Th17/Treg的比例;显著促进肠道SIgA的分泌;降低多种细胞因子的表达。结论氧化苦参碱通过靶向调控Th17分化改善环磷酰胺诱导的小鼠肠黏膜免疫屏障的损伤。
Objective To explore the protective effect of oxymatrine on the inhibition of Th17 differentiation on intestinal mucosal immune function injury in mice and initially explore its protection mechanism.Methods Thirty ICR mice were randomly divided into three groups:blank control group,injury control group and treatment group.A mouse model of intestinal mucosal damagewas induced by cyclophosphamide(CTX)injection.The changes in mental state,activity,villus length(V),crypt depth(C)and V/C ratio were detected.The percentage of Th17,Treg and Th17/Treg ratio in intestinal mucosal tissues were detected by Flow cytometry;The expression of RORγt and Foxp3 were detected by Western blot;SIgA contents in intestinal mucosa were detected by ELISA.Results Compared with the control group,V/C ratio and the secretion of SIgA in mice administered with oxymatrine were significantly improved,and the ratio of Th17/Treg in different periods and the expression of various were reduced.cytokines.Conclusion Oxymatrine can protect the intestinal mucosal immune function damage in mice from CTX.
作者
张岚琨
张志强
ZHANG Lankun;ZHANG Zhiqiang(School of Graduate,China Medical University,Shenyang 110000,China;People's Hospital of China Medical University,Shenyang 110000,China)
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2019年第12期1100-1106,共7页
Journal of Shenyang Pharmaceutical University
基金
沈阳市人口与健康科技计划项目(17-230-9-36).
