摘要
目的:通过建立栀黄消瘀止痛膏中大黄酚的含量测定方法,进行质量控制。方法:采用高效液相色谱(HPLC)法,色谱柱为岛津Wondasil C_(18)(4.6mm×250mm,5μm),流动相为甲醇-0.1%磷酸水溶液(80∶20),流速为1.0mL/min,检测波长为254nm,进样量为10μL,柱温为35℃。结果:大黄酚线性回归方程为:Y=44.098 X+0.5679,相关系数R2=1,表明大黄酚在4.068~65.08μg/mL浓度范围内进样量与峰面积呈良好的线性关系;精密度、重复性及稳定性试验相对标准偏差(RSD)分别为0.2%、1.1%、1.3%;大黄酚平均回收率为98.69%,RSD=1.2%≤2.0%。结论:HPLC法简便、灵敏、重复性好,可用于栀黄消瘀止痛膏中大黄酚的质量控制。
Objective:To establish a method for the determination of chrysophanol in Zhihuang Xiaoyu Zhitong Ointment.Methods:HPLC was used with Shimadzu wondasil C_(18)(4.6 mm×250 mm,5μm);the mobile phase was methanol-0.1%phosphoric acid solution(80∶20);the flow rate was 1.0 mL/min;the detection wavelength was 254 nm;the injection volume was 10μL;the column temperature was 35℃.Results:The linear regression equation of chrysophanol was y=44.098 x+0.5679,and the correlation coefficient was R2=1,indicating that there was a good linear relationship between the injection volume and the peak area in the concentration range of 4.068~65.08μg/mL;the relative standard deviations(RSD)of precision,repeatability and stability tests were 0.2%,1.1%and 1.3%,respectively;the average recovery of chrysophanol was 98.69%,RSD=1.2%≤2.0%.Conclusion:HPLC is simple,sensitive and reproducible,which can be used for the quality control of Chrysophanol in Zhihuang Xiaoyu Zhitong Ointment.
作者
鲁明明
林新艳
邬学群
莫文
许金海
范赵翔
汤茹茹
殷书梅
Lu Mingming;Lin Xinyan;Wu Xuequn;Mo Wen;Xu Jinhai;Fan Zhaoxiang;Tang Ruru;Yin Shumei(Jiangsu Kanion Sunshine Parmaceutical Co.Ltd,Nanjing 210046,China;Longhua Hospital Affiliated to Shanghai University of TCM,Shanghai 200032,Chnia)
出处
《亚太传统医药》
2021年第7期38-41,共4页
Asia-Pacific Traditional Medicine
基金
上海市科学技术委员会科研计划(17401901400)
