摘要
目的:重复-Gx加速度暴露对新西兰家兔心脏结构的影响。方法:20只新西兰家兔随机分为2组(n=10):对照组、-Gx加速度暴露组。-3.6 Gx暴露2 s,间隔5 min,每天重复20次,共30 d;对照组不受加速度作用。末次-Gx加速度暴露后,用静脉注射空气法处死动物,迅速取左心室心肌组织,常规取样并采用光学显微镜及透射电镜进行组织学观察。结果:-Gx加速度暴露组家兔心肌切片在光学显微镜下可见心肌细胞的形态及排列等与对照组无显著区别;-Gx加速度暴露组家兔心肌在透射电镜下可见,心肌纤维断裂、排列紊乱、心肌细胞水肿、核膜扩张、血管内皮基膜分离。结论:-Gx加速度暴露可造成家兔心肌细胞超微结构损伤,提示应重视长期重复-Gx加速度暴露对舰载战斗机飞行员心脏功能的影响和防护。
Objective:To observe the effects of repeated horizontal-Gx acceleration exposure on cardiac structure in New Zealand rabbits.Methods:Twenty New Zealand rabbits were divided into 2 groups(n=10):control group and-Gx acceleration exposure group.The rabbits in-Gx acceleration exposure group were exposed to-3.6 Gx with 2 s,at intervals of 5 min,repeated 20 times daily,with a total of 30 d;the control group didn’t undergo the acceleration stress.After the last-Gx acceleration exposure,the animals were killed by intravenous injection of air,and two small pieces of myocardium were immediately dissected from the left ventricles for structure examination using optical microscope and transmission electron microscope.Results:There was no significant difference in the myocardial cell morphology and arrangement observed under the optical microscope between the-Gx acceleration exposure group and the control group;the myocardial fibers arranged in disorder,myocardial cell edema,nuclear membrane expansion,vascular endothelial basement membrane separation were observed in the-Gx acceleration exposure group under transmission electron microscope,compared with the control group.Conclusion:-Gx acceleration exposure can lead to ultrastructural damage in rabbit cardiac myocytes.It suggested that the more attention should be paid to the effect and protection of long-term horizontal-Gx acceleration exposure on the cardiac function of carrier fighter pilots.
作者
周必业
李靖
李鸣皋
孙海文
周家兴
韩磊
ZHOU Bi-ye;LI Jing;LI Ming-gao△;SUN Hai-wen;ZHOU Jia-xing;HAN Lei(Center of Nautical and Aviation Medicine of PLA General Hospital,Beijing 100048,China)
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2020年第2期134-137,197,共5页
Chinese Journal of Applied Physiology
基金
全军重大科研项目(AHJ11Z001)
