摘要
探索醇沉处理前后中药水提液理化性质的变化及其与喷雾干燥黏壁的关系。该研究选取临床应用较多的中草药,分别测定其水提液醇沉处理前后的理化性质,并通过主成分分析(PCA-X),聚类分析(HCA),正交偏最小二乘-判别分析(OPLS-DA)等数据分析手段,深入挖掘药液醇沉处理与喷雾干燥热熔型黏壁的相关性。3种分析结果均显示醇沉处理影响了中药水提液的理化性质,且醇沉处理后中药药液的物理性质变化与喷雾干燥热熔型黏壁有显著相关性。PCA-X和HCA结果表明醇沉处理对中药水提液的动态表面张力影响最大,对其他5种理化性质也存在一定影响;OPLS-DA验证了PCA-X和HCA结果并显示醇沉处理对药液的动态表面张力、平衡表面张力、pH影响显著,影响程度由强到弱依次为动态表面张力>平衡表面张力>pH。因此,可以推测醇沉处理造成的药液动态表面张力和pH的下降是导致醇沉处理药液喷雾干燥出现热熔型黏壁现象的重要因素,可能是由于大分子醇不溶性成分的去除增大了小分子表面活性成分及有机酸、小分子糖的相对比例造成的。
To explore the relationship between the variations of the physiochemical properties of traditional Chinese medicine(TCM)decoction before or after precipitation in alcohol and the wall stickiness in spray drying.In this study,widely used TCMs in clinic were selected to determine the physiochemical properties of TCM decoction before or after precipitation in alcohol separately.Afterwards,the principle component analysis(PCA),Hierarchical cluster analysis(HCA),and orthogonal partial least squares-discriminate analysis(OPLS-DA)were used to evaluate the relationship between the variations of those liquid before or after precipitation in alcohol and hot-melt stickiness in spray drying.Three types of statistical analysis methods all indicated that ethanol precipitation affected physiochemical properties of TCM decoction,and the variations of physical properties showed significant association with hot-melt stickiness in spray drying.The results of PCA-X and HCA suggested that the dynamic surface tension(DST)was impacted most by the alcohol deposition treatment,at the same time,the other 5 physiochemical properties were also affected.OPLS-DA verified that PCA-X and HCA results,and revealed that DST,equilibrium surface tension(EST)and pH were significantly affected by alcohol deposition treatment,and the order of the affecting factors from high to low was DST,EST and pH.Therefore,the downward trend of DST and pH were the important factors that directly affected the hot-melt stickiness of TCM after precipitation in alcohol,which would be probably caused by losing macromolecules alcoholic insoluble components and increasing relative proportions of organic acid and small molecule sugar.
作者
施晓虹
杨日昭
赵立杰
洪燕龙
冯怡
王优杰
SHI Xiao-hong;YANG Ri-zhao;ZHAO Li-jie;HONG Yan-long;FENG Yi;WANG You-jie(Engineering Research Center of Modern Preparation Technology of Traditional Chinese Medicine under Ministry of Education,Innovation Research Institute of TCM,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shanghai Innovation Center of Traditional Chinese Medicine Health Service,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2020年第4期846-853,共8页
China Journal of Chinese Materia Medica
基金
上海市科学技术委员会项目(16401902300)
上海市卫计委中医药科技创新项目(ZYKC201603003).
关键词
水提醇沉
喷雾干燥
热熔型黏壁
动态表面张力
water extraction and alcohol precipitation
spray drying
hot-melt stickiness
dynamic surface tension
