摘要
目的建立高效液相色谱法(HPLC)梯度洗脱法同时测定补血调经片中甘草素、美迪紫檀素、香附烯酮和α-香附酮含量。方法采用Waters Symmetry C18色谱柱(250 mm×4.6 mm,5μm);流动相乙腈-0.1%磷酸溶液,梯度洗脱,体积流量1.0 ml/min;柱温30℃,检测波长分别为280 nm(甘草素、美迪紫檀素)和242 nm(香附烯酮、α-香附酮)。结果甘草素、美迪紫檀素、香附烯酮和α-香附酮分别在1.09~21.80、1.26~25.20、19.58~391.60、8.91~178.20μg/ml范围内线性关系良好(r≥0.9992),平均加样回收率分别为96.96%、98.88%、99.85%、99.53%,相对标准偏差(RSD)分别为1.03%、1.17%、0.83%和0.77%。结论HPLC梯度洗脱法准确可靠,可用于补血调经片的质量控制。
Objective To establish an HPLC gradient elution method for simultaneous determination of liquiritigenin,medicarpin,cyperotundone andα-cyperone in Buxue Tiaojing Tablets.Methods The chromatographic separation was carried out on a Waters Symmetry C18(250 mm×4.6 mm,5μm)column,with mobile phase composed of acetonitrile-0.1%phosphoric acid solution as a mobile phase in gradient elution.The flow rate was 1.0 mL·min-1,the column temperature was 30℃.The detection wavelength were 280 nm for liquiritigenin and medicarpin,and 242 nm for cyperotundone andα-cyperone.Results Liquiritigenin,medicarpin,cyperotundone andα-cyperone showed good linear relationships within the ranges of 1.09-21.80,1.26-25.20,19.58-391.60,8.91-178.20μg·mL-1(r≥0.9992),whose average recoveries were 96.96%,98.88%,99.85%,99.53%with the RSDs of 1.03%,1.17%,0.83%,0.77%,respectively.Conclusion HPLC Gradient elution method is accurate and reliable,which can be used for the quality control of Buxue Tiaojing Tablets.
作者
许伟
XU Wei(Dongying Food and Drug Inspection Center,Dongying 257091,China)
出处
《中国药物经济学》
2020年第2期37-40,共4页
China Journal of Pharmaceutical Economics
关键词
补血调经片
梯度洗脱
多指标
甘草素
美迪紫檀素
香附烯酮
Α-香附酮
Buxue Tiaojing Tablet
Gradient elution
Multipleindicators
Liquiritigenin
Medicarpin
Cyperotundone
α-cyperone
