摘要
〔目的〕了解大连口岸蚊虫种类、生态习性及淡色库蚊、三带喙库蚊自然感染流行性乙型脑炎病毒情况。〔方法〕用帐诱法、灯诱法、采集幼虫等方法进行调查,并应用逆转录-聚合酶联反应技术(RT-PCR)进行乙脑病毒鉴定。〔结果〕大连口岸捕获蚊虫3属9种,其中按蚊属1种,伊蚊属4种,库蚊属4种。大连口岸蚊虫于5月出现,8月达到高峰,9月逐渐减少,10月消失。侵袭人类以淡色库蚊(占47.98%)、东乡伊蚊(占12.15%)和背点伊蚊(占10.41%)为多。不同场所蚊虫密度差异反映出蚊虫的不同嗜血习性,淡色库蚊、凶小库蚊和白纹伊蚊喜嗜人血;淡色库蚊同时喜嗜猪、牛血;三带喙库蚊偏嗜猪血;中华按蚊偏嗜牛血;背点伊蚊和刺扰伊蚊偏嗜猪、牛血。2007年8月中旬首先在三带喙库蚊中检出乙脑病毒,共检测70批次样本,阳性率为4.28%。〔结论〕淡色库蚊、东乡伊蚊、背点伊蚊是该地区危害人类的主要蚊种。大连口岸蚊虫自然感染乙脑病毒于8月中下旬开始,三带喙库蚊首先染毒。本调查为蚊虫防制提供了生态学依据,为预报流行性乙型脑炎的发生时间和流行强度提供了科学依据。
Objective To Investigate the community structure of mosquito and to study natural infection of the encephalitis B virus of Culex pipiens pallens and Culex tritaeniorhynchus at Dalian port. Method Human baited net trap method,lamp baited trap method ,larvae collection method were adopted. Meanwhile, the virus was identified wiih RT-PCR. Result Mosquitoes were collected at Dalian port, meanwhile they were subjected to 3 genera 9 species. In which, one species of Anopheles ,four species of Aedes ,four species of Culex.Mosquitoes came forth in May,reached the peak in Augest,declined gradually from September and disappeared in October.Human were attacked mainly by Culex pipiens pallens（47.98%）,Ae.（F.） togoi （12.15%） and Ae. （0.） dorsalis （10.41%）.The density in different occasion reflected the various features of mosquitoes, Culex pipiens pallens, C.（Ba） modestus,Ae （S.） albopictus profer human blood, C.（C.） pipiens pallens also profer pig and cow blood, C.（C.） tritaeniorhynchus profer pig blood,An.（A.）sinensis profer cow blood, Ae.（O.） dorsalis and Ae.（Aedimorphus） vexans profer pig and cow blood.The encephalitis B virus was first found on Augest,2007. Of the 70 groups of samples were examined,the positive rate was 4.28%. Conclusion Culex pipiens pallens ,Ae.（F.） togoi and Ae.（O.） dorsalis are dominant population in this area.The natural infection of encephaitis B virus of mosquitoes begin in mid Augest.Culex tritaeniorhynchus is the first infected species.The investigation have provided some basic foundations for mosquito control,meanwhile, can provide scientific support for the prediction of the happening time and prevalence intension of the epidemic encephalitis B virus.
出处
《中国国境卫生检疫杂志》
CAS
2009年第5期363-367,共5页
Chinese Journal of Frontier Health and Quarantine
