摘要
目的研究miR-214和磷酸酶及张力蛋白同源物(PTEN)在幽门螺杆菌(HP)感染的胃癌组织中的表达及意义。方法选取2016年6月—2019年6月在徐州市第一人民医院接受治疗的HP感染的胃癌患者66例为研究对象。采用实时荧光定量PCR(qRT-PCR)法检测胃癌组织和癌旁组织中miR-214水平。采用免疫组化法测定胃癌组织和癌旁组织中PTEN表达水平。分析miR-214和PTEN表达水平与胃癌患者临床病理特征的关系。术后随访,探究miR-214和PTEN表达水平与患者预后的关系。结果与癌旁组织相比,胃癌组织miR-214表达水平较高(P<0.05),PTEN阳性表达率较低(P<0.05)。miR-214、PTEN与TNM分期、有无远处转移有关(P<0.05),与年龄、性别、肿瘤直径无关(P>0.05)。miR-214与PTEN呈显著负相关(P<0.05)。miR-214高表达胃癌患者平均生存时间显著短于miR-214低表达胃癌患者(P<0.05),PTEN阴性胃癌患者平均生存时间显著短于PTEN阳性胃癌患者(P<0.05)。结论HP感染的胃癌组织中miR-214呈高表达,PTEN阳性表达率低,miR-214和PTEN表达与HP感染的胃癌患者预后情况密切相关。
Objective To study the expression and significance of miR-214,PTEN in gastric cancer tissues infected with Helicobacter pylori(HP).Methods A total of 66 patients with gastric cancer infected with HP who were treated at the First People′s Hospital of Xuzhou from June 2016 to June 2019 were selected.Real-time fluorescence quantitative PCR(qRT-PCR)was used to detect the levels of miR-214 and immunohistochemistry was employed to determine the expression of PTEN in gastric cancer tissues and adjacent tissues.The relationship between the levels of miR-214 and PTEN and the clinical-pathological features of gastric cancer patients was analyzed.After postoperative follow-up,the relationship between miR-214 and PTEN levels and the prognosis of the patients was explored.Results Compared with adjacent tissues,gastric cancer tissues showed higher levels of miR-214(P<0.05)and lower positive expression rates of PTEN(P<0.05).MiR-214 and PTEN were associated with TNM stage and distant metastasis(P<0.05)but not with age,gender,or tumor diameter(P>0.05).MiR-214 was significantly negatively correlated with PTEN(P<0.05).The mean survival time of patients with high expression of miR-214 was significantly shorter than that of patients with low expression of miR-214(P<0.05).Gastric cancer patients with negative PTEN expression had significantly shorter average survival time than those with positive PTEN expression(P<0.05).Conclusions MiR-214 is highly expressed in gastric cancer tissues infected with HP,and PTEN has a low positive expression rate.The expression of miR-214 and PTEN is closely related to the prognosis of gastric cancer patients infected with HP.
作者
高之峰
李慧
王庄梅
GAO Zhifeng;LI hui;WANG Zhuangmei(Department of Gastroenterology,the First People′s Hospital of Xuzhou,Xuzhou,Jiangsu 221109,China)
出处
《徐州医科大学学报》
CAS
2023年第10期751-755,共5页
Journal of Xuzhou Medical University
基金
江苏省科技项目(BE2019688)。
关键词
miR-214
磷酸酶及张力蛋白同源物
胃癌
幽门螺杆菌
感染
miR-214
phosphatase and tensin homology deleted on chromosome ten
gastric cancer
Helicobacter pylori
infection
