摘要
目的探讨miR-214在胃癌中的表达及临床意义。方法采用实时荧光定量PCR法,检测40例胃癌患者癌组织及癌旁组织中miR-214的差异表达;同时检测血浆miR-214在56例胃癌患者及40例正常组织中的表达。结果miR-214在胃癌组织中的表达水平明显高于癌旁组织;与正常组织比较,胃癌患者中的血浆miR-214表达明显增高;血浆miR-214表达与患者性别、年龄无关,与疾病分期及生存期密切相关,差异具有统计学意义(P<0.05);Cox回归分析发现疾病分期、miR-214的表达可作为独立的预后因子(P<0.05)。结论循环miR-214表达与胃癌肿瘤分期和生存时间相关,检测循环miR-214可能作为评估胃癌患者临床预后的靶基因。
Objective To analyze the expression of miR-214 in gastric cancer,and its clinical significance. Methods The expression of miR-214 were confirmed in primary gastric cancer tissues and para-earcinoma tissues by QRT-PCR;Meanwhile, the expression of plasma miR-214 were detected in 56 consecutive gastric cancer patients and 40 healthy volunteers and the clini- cal significance was evaluated. Results Expression of miR-214 was significantly higher in gastric cancer tissues than in para-car- cinoma tissues;Plasma miR-214 concentrations were significantly higher in gastric cancer patients than that in normal people （P 〈 0.05 ） ;The expression of plasma miR-214 were not associated with gender, and age（ P 〉 0.05 ）, but significantly correlated with overall survival and clinical stage（ P 〈 0. 001 ）. Cox regression analysis found that plasma miR-214 expression and disease stage were independent prognostic factors for the gastric cancer patients. Conclusion Plasma miR-214 is related with clinical stage and survival of gastric cancer,it could be a useful biomarker for evaluation of prognosis of gastric cancer.
出处
《实用癌症杂志》
2017年第6期894-899,共6页
The Practical Journal of Cancer
