摘要
目的在体内外实验中探讨中药组合物夏丹芪(冬虫夏草、丹参、黄芪)对小鼠氡暴露肺组织损伤的防治效果。方法建立氡暴露小鼠模型,分为健康对照组、氡暴露组和夏丹芪干预氡暴露组,每组6只。HE和Masson染色观察120 WLM时各组小鼠肺组织病理变化,免疫组织化学法检测肺组织α-SMA蛋白和Vimentin蛋白表达;超氧化物歧化酶(SOD)和丙二醛(MDA)试剂盒检测各组肺组织氧化应激水平。同时,利用生态氡室构建氡暴露细胞模型和氡暴露+夏丹芪干预细胞模型,粘附试剂盒检测不同组细胞粘附能力;Transwell迁移实验确定各组细胞迁移能力;Western blot法检测各组细胞E-cadherin和Vimentin蛋白的表达情况。结果与氡暴露组相比,2 mg/g夏丹芪干预可使氡暴露小鼠肺组织MDA浓度降低(t=4.43,P<0.05),SOD活性升高(t=3.22,P<0.05);肺组织α-SMA和Vimentin蛋白表达降低(t=3.08、7.57,P<0.05)。在体外实验中,与单纯氡暴露组相比,150和200μg/ml夏丹芪干预后氡暴露细胞迁移能力下降(t=4.78、13.01,P<0.05),细胞粘附性能增强(t=3.41、12.55,P<0.05);E-cadherin蛋白表达增高(t=2.96、19.57,P<0.05),Vimentin蛋白表达明显下降(t=21.00、33.32,P<0.05)。结论中药组合物夏丹芪可通过降低辐射诱发的氧化应激、减弱氡暴露诱发的上皮细胞间质转化和纤维化,对氡暴露损伤具有一定的辐射防护作用,有望成为氡损伤的辐射防护剂。
Objective To explore the effect of a Chinese medicinal composition(Xiadanqi)on the prevention of radon exposure induced injuries of lung in vitro and in vivo.Methods Mice were randomly divided into three groups of blank control group,radon-exposed group alone and radon-exposed group intervened with Chinese medicinal composition.The pathological changes of lung tissues in each group after 120 WLM were observed by HE and Masson staining,and the expressions ofα-SMA protein and Vimentin protein in lung tissues were detected by immunohistochemistry staining.The levels of oxidative stress in lung tissue of each group were detected with SOD and MDA kits.At the same time,a radon exposed cell model and a radon exposure+Xiadanqi intervention cell model were constructed using an ecological radon chamber.The cell adhesion abilities of different groups were detected by an adhesion kit.The cell migration ability of each group was determined by the transwell migration experiment.The expression of E-cadherin and Vimentin protein was detected by Western blot.Results Compared with the radon exposure group,the concentration of MDA was decreased(t=4.43,P<0.05),the activity of SOD was increased(t=3.22,P<0.05),andα-SMA and Vimentin protein expressions were decreased(t=3.08,7.57,P<0.05)in lung tissue of mice intervened with 2 mg/g Xiadanqi.In vitro,compared with radon exposure group,the migration ability was reduced(t=4.78,13.01,P<0.05),the cell adhesion property was enhanced(t=3.41,12.55,P<0.05),the expression of E-cadherin protein was increased(t=2.96,19.57,P<0.05),and the expression of Vimentin protein was obviously reduced(t=21.00,33.32,P<0.05)in radon-exposed cells with the treatment of Chinese medicine(150μg/ml and 200μg/ml).Conclusions The Chinese medicinal composition(Xiadanqi)has a certain radioprotective effect on radon exposure induced injury by reducing oxidative stress,attenuating EMT and fibrosis,and thus it may be applied as a protective agent for radon induced injury.
作者
黎晏卿
江其生
史更生
田思琦
张蓉
程菲
崔凤梅
涂彧
陈慧芹
Li Yanqing;Jiang Qisheng;Shi Gengsheng;Tian Siqi;Zhang Rong;Cheng Fei;Cui Fengmei;Tu Yu;Chen Huiqin(State Key Laboratory of Radiation Medicine and Protection,School for Radiological and Interdisciplinary Sciences(RAD-X),Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions,Soochow University,Suzhou 215123,China;PLA Rocket Characteristic Medical Center,Beijing 100088,China)
出处
《中华放射医学与防护杂志》
CSCD
北大核心
2021年第8期587-594,共8页
Chinese Journal of Radiological Medicine and Protection
基金
军队后勤研究项目(AEP17J001)
江苏省大学生创新创业训练项目(201910285045Z)。
关键词
氡暴露
中药组合物
支气管上皮细胞
辐射防护
Radon exposure
Chinese medicinal composition
Bronchial epithelial cells
Radiation protection
