摘要
建立了高效液相色谱-串联质谱法（HPLC-MS/MS）同时测定食用香精中的四种黄曲霉素类真菌毒素的方法。食用香精样品用甲醇-水（84∶16,v/v）萃取,提取液经ODS C18分散固相萃取净化,采用HPLC-MS/MS进行色谱柱分析。待测物标准品在0.1~30μg/L浓度范围内线性关系良好,相关系数r〉0.991;B1、B2、G1和G2定量限（LOQ）分别为0.28、0.36、0.41和0.22μg/kg,回收率在61.4%~116.9%之间,相对标准偏差（RSD）在4.5%~11.9%之间。结果表明,该方法灵敏度高、选择性强且稳定性好,可用于食用香精中黄曲霉素类真菌毒素的检测。
An easy method for the determination of afl atoxin mycotoxins in edible essence by high performance liquid chromatography coupled with tandem mass spectrometry（HPLC-MS/MS）has been developed. Edible essence samples were extracted with methanol-water（84 ∶ 16,v/v）and then it was purified by ODS C18 matrix solidphase dispersion,and the extracts were determined by HPLC-MS/MS. The good correlation coefficients（r 0.991）of the four aflatoxins were obtained within their respective linear ranges（0.1 ~ 30μg/L）. The determination limits for 4 aflatoxins were 0.28、 0.36、 0.41、0.22 μg/kg,the average recoveries were between 61.4 % ~ 116.9%,the relative standard deviations（RSD）were in the range from 4.5% to11.9 %. The results indicated that the developed method is sensitive,fast,accurate,and can be applied for the determination of the aflatoxin class mycotoxins in edible essence samples.
出处
《中国食品添加剂》
CAS
北大核心
2016年第6期180-186,共7页
China Food Additives
