摘要
目的尽快明确艾滋病病毒Ⅰ型(HIV-1)抗体确证实验"不确定"样本的结果,以便相关检测目的人员尽早知晓HIV感染状况。方法对2007-2013年确证结果为"不确定"的样本,与初筛实验的两种酶联免疫吸附试验(ELISA)和两种快诊的结果进行比对分析,并对确证实验条带本身作分析总结。结果 78例"不确定-阴性"样本,经两种ELISA及两种快诊初筛实验,至少一种实验为阴性;38例"不确定-阳性"样本,经两种ELISA和两种快诊均为阳性。确证时同时出现gp160和gp120两条或以上条带,"不确定"结果经随访最终均转为"阳性";出现gp160/gp160和p24条带,"不确定"结果经随访最终转归具不确定性;出现除gp160条带的其余单独条带或组合条带的"不确定"结果,经随访最终均转为"阴性"。结论两种ELISA和两种快诊初筛均为阳性的样本,确证实验"不确定"结果的"阳性"转归率为100%;但只要其中一种实验结果为阴性,"阳性"转归率均为0。建议对于一些确证条带疑似"阳性"的样本,如同时出现gp160和gp120两条以上带型者,应结合初筛实验结果综合分析,尽早明确诊断。
Objective To determine outcome of uncertain results in HIV-1 antibody confirmatory tests, for health workers to know the status of HIV infection as soon as possible. Method The uncertain samples of confirmatory tests in 2007-2013 were retested using two kinds of ELISA screening test and two kinds of rapid HIV test, and the results were analyzed with the bands in confirmatory test. Results Of 78 cases of ＂uncertain-negative＂ samples, at least one test was negative among two ELISA and two kinds of quick diagnostic screening experiments. Of 38 cases of ＂ uncertain - positive＂ samples, the two ELISA and the two quick diagnostic tests were all positive. If gpl60and gpl20 or more bands were simultaneously present in the confirmatory test, ＂ uncertain＂ results would eventually turned into positive during follow-up. If band（s） of gp160 or gp160 and p24 occurred, the outcome of ＂ uncertain＂ results were uncertain during follow-up. If single band or combination bands except gpl60 occurred, the ＂uncertain＂ results would eventually turned into negative during follow-up. Conclusion If an uncertain samples in the confirmatory test were positive in two ELISA and two kinds of quick diagnostic tests, its seroconversion rate would be 100%. But as long as one of the tests were negative, the seroconversion rate would he 0. For some suspected positive samples in the confirmatory test, if gp160 and gp120 or more bands occur, comprehensive analysis should be performed combined with screening test results for early diagnosis.
出处
《中国艾滋病性病》
CAS
2014年第11期866-868,共3页
Chinese Journal of Aids & STD
